Colorimetric PCR-Based microRNA Detection Method Based on Small Organic Dye and Single Enzyme
文献类型：期刊论文
| 作者 | Dong, Juan2; Chen, Gangyi2; Wang, Wei2; Huang, Xin2; Peng, Huipan2; Pu, Qinlin2; Du, Feng2; Cui, Xin2; Deng, Yun1; Tang, Zhuo2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2019-06-19 |
| 卷号 | 90期号:12页码:7107-7111 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b01111 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | microRNAs (miRNAs) have been a class of promising disease diagnostic biomarkers and therapeutic targets for their important biological functions. However, because of the high homology, interference from precursors (pri-miRNA, pre-miRNA), as well as limitations in the current assay technologies, it poses high demand and challenge for a specific, efficient, and economic miRNA assay method. Here, we propose a new miRNA detection method based on a label-free probe and a small organic dye with sequence dependence, realizing the sequence-specific and colorimetric detection of target miRNA. What is pleasantly surprising, only one enzyme is enough to propel the whole miRNA assay process, greatly simplifying the reaction component and detection process. Together with PCR amplification for the high enough sensitivity a nd three checks for specificity control, a detection limit of 5 fM was obtained and even one mutation could be discriminated visually. Overall, the new method makes much progress in convenience and economy of PCR-based miRNA assay method so that miRNA assay is going to be more friendly and affordable. |
| 学科主题 | Chemistry ; Analysis |
| URL标识 | 查看原文 |
| WOS关键词 | HYBRIDIZATION CHAIN-REACTION ; REVERSE-TRANSCRIPTASE ACTIVITY ; LABEL-FREE ; ISOTHERMAL AMPLIFICATION ; SIGNAL AMPLIFICATION ; BIOSENSING STRATEGY ; DNA-POLYMERASE ; CANCER ; NANOPARTICLES ; ASSAY |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000436028800002 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://210.75.237.14/handle/351003/30227]  |
| 专题 | 国家天然药物工程技术研究中心_天然产物研究 |
| 作者单位 | 1.Chengdu Univ TCM, State Key Lab Breeding Base Systemat Res Dev & Ut, Chengdu 611137, Sichuan, Peoples R China 2.Chinese Acad Sci, Nat Prod Res Ctr, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Dong, Juan,Chen, Gangyi,Wang, Wei,et al. Colorimetric PCR-Based microRNA Detection Method Based on Small Organic Dye and Single Enzyme[J]. ANALYTICAL CHEMISTRY,2019,90(12):7107-7111. |
| APA | Dong, Juan.,Chen, Gangyi.,Wang, Wei.,Huang, Xin.,Peng, Huipan.,...&Tang, Zhuo.(2019).Colorimetric PCR-Based microRNA Detection Method Based on Small Organic Dye and Single Enzyme.ANALYTICAL CHEMISTRY,90(12),7107-7111. |
| MLA | Dong, Juan,et al."Colorimetric PCR-Based microRNA Detection Method Based on Small Organic Dye and Single Enzyme".ANALYTICAL CHEMISTRY 90.12(2019):7107-7111. |
入库方式： OAI收割
来源：成都生物研究所
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