Evolution of microbial biosensor based on functional RNA through fluorescence-activated cell sorting
文献类型：期刊论文
| 作者 | Li, Na; Huang, Xin; Zou, Jiawei; Chen, Gangyi; Liu, Getong; Li, Mei; Dong, Juan; Du, Feng; Cui, Xin; Tang, Zhuo |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2019-04-01 |
| 卷号 | 258期号:2018页码:550-557 |
| 关键词 | Whole-cell Biosensor Aptamer RNA Spinach FACS |
| ISSN号 | 0925-4005 |
| DOI | 10.1016/j.snb.2017.11.015 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | Whole-cell microbial biosensors are emerging as tools for rapid, economic and on-site detection. Anew approach to construct microbial biosensor with functional RNA for sensing of specific molecules is described here. The sensor is based on Spinach, an RNA mimic of GFP, and transcribed in living bacteria to detect intended molecules through fluorescent signals. Based on high-throughput cell cytometry, microbes with high selectivity and sensitivity could be screened out and evolved. Three microbial biosensors obtained through this method can efficiently detect tobramycin in different biosamples, providing a simple and economic solution for both qualitative and quantitative detection of antibiotics in the complex environments. This new strategy for the construction of whole-cell microbial biosensor and in vivo evolution protocol is especially suitable for any exogenous targets able to enter into bacteria and does not cause bacterial death in a short time, not just intracellular substances. (c) 2017 Elsevier B.V. All rights reserved. |
| 学科主题 | Spectroscopy ; Instrumentation ; Analytical Sciences |
| URL标识 | 查看原文 |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; AMINOGLYCOSIDE ANTIBIOTICS ; SYNTHETIC BIOLOGY ; RESIDUES ; APTAMER ; RIBOSWITCH ; BINDING ; PROTEIN ; TRANSLATION |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:000424868700063 |
| 出版者 | ELSEVIER SCIENCE SA |
| 源URL | [http://210.75.237.14/handle/351003/30295]  |
| 专题 | 国家天然药物工程技术研究中心_天然产物研究 |
| 作者单位 | Univ Chinese Acad Sci, Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Sichuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Na,Huang, Xin,Zou, Jiawei,et al. Evolution of microbial biosensor based on functional RNA through fluorescence-activated cell sorting[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,258(2018):550-557. |
| APA | Li, Na.,Huang, Xin.,Zou, Jiawei.,Chen, Gangyi.,Liu, Getong.,...&Tang, Zhuo.(2019).Evolution of microbial biosensor based on functional RNA through fluorescence-activated cell sorting.SENSORS AND ACTUATORS B-CHEMICAL,258(2018),550-557. |
| MLA | Li, Na,et al."Evolution of microbial biosensor based on functional RNA through fluorescence-activated cell sorting".SENSORS AND ACTUATORS B-CHEMICAL 258.2018(2019):550-557. |
入库方式： OAI收割
来源：成都生物研究所
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