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Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8

文献类型：期刊论文

作者He, Tian Tian2,3; Zhou, Ying2,3; Liu, Ying Li2,3; Li, Duan You2,3; Nie, Pin1,2,4; Li, Ai Hua2; Xie, Hai Xia2
刊名CELLULAR MICROBIOLOGY
出版日期2020-07-01
卷号22期号:7页码:16
关键词apoptosis Edwardsiella piscicida type 1 fimbriae type III protein EseJ
ISSN号1462-5814
DOI10.1111/cmi.13193
通讯作者Li, Ai Hua(liaihua@ihb.ac.cn) ; Xie, Hai Xia(xieh@ihb.ac.cn)
英文摘要The type III secretion system effector EseJ plays a regulatory role inside bacteria. It suppresses the adherence of Edwardsiella piscicida (E. piscicida) to host epithelial cells by down regulating type 1 fimbriae. In this study, we observed that more macrophages infected with Delta eseJ strain of E. piscicida detached as compared with those infected with the wild-type (WT) strain. Terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) staining and cleaved caspase-3 examination revealed that the detachment is due to increased apoptosis, suggesting that EseJ suppresses macrophage apoptosis. However, apoptosis inhibition by EseJ is not relative to a type III secretion system (T3SS) and is not related to EseJ's translocation. Since EseJ negatively regulates type 1 fimbriae, murine J774A.1 cells were infected with Delta eseJ Delta fimA or Delta eseJ Delta fimH strains. It was demonstrated that Delta eseJ stimulates macrophage apoptosis through type 1 fimbriae. Moreover, we found that infecting J774A.1 cells with the Delta eseJ strain increased levels of cleaved caspase-8, caspase-9, and caspase-3, demonstrating that EseJ inhibits apoptosis through either an extrinsic or a combination of extrinsic and intrinsic pathways. Pre-treatment of macrophages with caspase-8 inhibitor prior to infection with the Delta eseJ strain decreased the levels of cleaved caspase-8, caspase-9, and caspase-3, indicating that the Delta eseJ strain stimulates apoptosis, mainly through an extrinsic pathway by up regulating type 1 fimbriae. Zebrafish larvae or blue gourami fish infected with the Delta eseJ strain consistently exhibited higher apoptosis than those infected with the E. piscicida WT strain or Delta eseJ Delta fimA strain. Taken together, we revealed that the T3SS protein EseJ of E. piscicida inhibits host apoptosis, mainly through an extrinsic pathway by down regulating type 1 fimbriae.
WOS关键词SECRETION SYSTEM ; CHROMOSOMAL GENES ; CELL-DEATH ; TARDA ; IDENTIFICATION ; EFFECTOR ; ACTIVATION ; CLUSTER
WOS研究方向Cell Biology ; Microbiology
语种英语
WOS记录号WOS:000540798600006
出版者WILEY
源URL[http://ir.ihb.ac.cn/handle/342005/36817]  
专题水生生物研究所_中科院水生所知识产出（2009年前）
通讯作者Li, Ai Hua; Xie, Hai Xia
作者单位1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 He, Tian Tian,Zhou, Ying,Liu, Ying Li,et al. Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8[J]. CELLULAR MICROBIOLOGY,2020,22(7):16.
APA He, Tian Tian.,Zhou, Ying.,Liu, Ying Li.,Li, Duan You.,Nie, Pin.,...&Xie, Hai Xia.(2020).Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8.CELLULAR MICROBIOLOGY,22(7),16.
MLA He, Tian Tian,et al."Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8".CELLULAR MICROBIOLOGY 22.7(2020):16.

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来源：水生生物研究所

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