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  5. 期刊论文
Specific cyprinid HIF isoforms contribute to cellular mitochondrial regulation

文献类型：期刊论文

作者Chen, Jing1,4; Guan, Lihong3; Zou, Ming1,4; He, Shunping2; Li, Dapeng1,4; Chi, Wei1,4
刊名SCIENTIFIC REPORTS
出版日期2020-10-14
卷号10期号:1页码:13
ISSN号2045-2322
DOI10.1038/s41598-020-74210-w
通讯作者Chi, Wei(bluetorry@163.com)
英文摘要Hypoxia-inducible factor 1 (HIF-1) functions as a master regulator of the cellular response to hypoxic stress. Two HIF-1 alpha paralogs, HIF-1 alpha A and HIF-1 alpha B, were generated in euteleosts by the specific, third round of genome duplication, but one paralog was later lost in most families with the exception of cyprinid fish. How these duplicates function in mitochondrial regulation and whether their preservation contributes to the hypoxia tolerance demonstrated by cyprinid fish in freshwater environments is not clear. Here we demonstrated the divergent function of these two zebrafish Hif-1a paralogs through cellular approaches. The results showed that Hif-1aa played a role in tricarboxylic acid cycle by increasing the expression of Citrate synthase and the activity of mitochondrial complex II, and it also enhanced mitochondrial membrane potential and ROS production by reducing free Ca2+ in the cytosol. Hif-1ab promoted intracellular ATP content by up-regulating the activity of mitochondrial complexes I, III and IV and the expression of related genes. Furthermore, both the two zebrafish Hif-1a paralogs promoted mitochondrial mass and the expression level of mtDNA, contributing to mitochondrial biogenesis. Our study reveals the divergent functions of Hif-1aa and Hif-1ab in cellular mitochondrial regulation.
WOS关键词WHOLE-GENOME DUPLICATION ; OXIDATIVE-PHOSPHORYLATION ; ELECTRON-TRANSPORT ; DYSFUNCTION ; BIOGENESIS ; EVOLUTION ; CA2+ ; ROS ; INHIBITION ; METABOLISM
资助项目National Natural Science Foundation of China[31401977] ; earmarked fund for China Agriculture Research System[CARS-45] ; Fundamental Research Funds for the Central Universities[2662015PY119]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000582678700040
出版者NATURE RESEARCH
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
源URL[http://ir.ihb.ac.cn/handle/342005/39218]  
专题水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文
通讯作者Chi, Wei
作者单位1.Hubei Prov Engn Lab Pond Aquaculture, Wuhan, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
3.Xinxiang Med Univ, Coll Life Sci & Technol, Xinxiang, Henan, Peoples R China
4.Huazhong Agr Univ, Coll Fisheries, Natl Demonstrat Ctr Expt Aquaculture Educ, Wuhan 430070, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Jing,Guan, Lihong,Zou, Ming,et al. Specific cyprinid HIF isoforms contribute to cellular mitochondrial regulation[J]. SCIENTIFIC REPORTS,2020,10(1):13.
APA Chen, Jing,Guan, Lihong,Zou, Ming,He, Shunping,Li, Dapeng,&Chi, Wei.(2020).Specific cyprinid HIF isoforms contribute to cellular mitochondrial regulation.SCIENTIFIC REPORTS,10(1),13.
MLA Chen, Jing,et al."Specific cyprinid HIF isoforms contribute to cellular mitochondrial regulation".SCIENTIFIC REPORTS 10.1(2020):13.

入库方式： OAI收割

来源：水生生物研究所

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