Elovl2 But Not Elovl5 Is Essential for the Biosynthesis of Docosahexaenoic Acid (DHA) in Zebrafish: Insight from a Comparative Gene Knockout Study
文献类型：期刊论文
| 作者 | Liu, Chengjie1,2; Ye, Ding1,2; Wang, Houpeng2; He, Mudan1,2; Sun, Yonghua1,2 |
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2020-09-03 |
| 页码 | 7 |
| 关键词 | Elovl2 Elovl5 Docosahexaenoic acid Zebrafish |
| ISSN号 | 1436-2228 |
| DOI | 10.1007/s10126-020-09992-1 |
| 通讯作者 | Sun, Yonghua(yhsun@ihb.ac.cn) |
| 英文摘要 | Teleost fish can synthesize one of the major omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFAs), docosahexaenoic acid (DHA, 22:6n-3), from dietary alpha-linolenic acid (ALA; 18:3n-3), via elongase of very long-chain fatty acid (Elovl) and fatty acid desaturase (Fads). However, it remains unclear which elongase is primarily responsible for the endogenous synthesis of DHA. Here, in this study, the knockout models of the two major elongases, Elovl2 and Elovl5, were generated by CRISPR/Cas9 approach in zebrafish and comparatively analyzed. The homozygous mutants were validated by Sanger sequencing, mutation-mediated PCR, and whole-mount in situ hybridization analysis of the endogenous target genes. Compared with wild-type (WT) counterparts, the content of DHA was significantly reduced by 67.1% (P < 0.05) in the adult liver and by 91.7% (P < 0.01) in the embryo at 3-day post-fertilization (dpf) of theelovl2mutant, but not of theelovl5mutant. Further study revealed thatelovl2andfads2was upregulated by 9.9-fold (P < 0.01) and 9.7-fold (P < 0.01) in theelovl5mutant, andelovl5andfads2were upregulated by 15.1-fold (P < 0.01) and 21.5-fold (P < 0.01) in theelovl2mutant. Our study indicates that although both Elovl2 and Elovl5 have the elongase activity toward C20, the upregulation ofelovl2could completely replace the genetic depletion ofelovl5, but upregulation ofelovl5could not compensate the endogenous deficiency ofelovl2in mediating DHA synthesis. In conclusion, the endogenous synthesis of DHA in is mediated by Elovl2 but not Elovl5 in zebrafish and a DHA-deficient genetic model of zebrafish has been generated. |
| WOS关键词 | POLYUNSATURATED FATTY-ACIDS ; LONG-CHAIN PUFA ; ELONGATION ; NUCLEASE |
| 资助项目 | National Key R&D Program of China[2018YFA0801000] ; National Natural Science Foundation of China[31972780] ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology[2019FBZ05] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000565473900001 |
| 出版者 | SPRINGER |
| 资助机构 | National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/38631]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 通讯作者 | Sun, Yonghua |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Chengjie,Ye, Ding,Wang, Houpeng,et al. Elovl2 But Not Elovl5 Is Essential for the Biosynthesis of Docosahexaenoic Acid (DHA) in Zebrafish: Insight from a Comparative Gene Knockout Study[J]. MARINE BIOTECHNOLOGY,2020:7. |
| APA | Liu, Chengjie,Ye, Ding,Wang, Houpeng,He, Mudan,&Sun, Yonghua.(2020).Elovl2 But Not Elovl5 Is Essential for the Biosynthesis of Docosahexaenoic Acid (DHA) in Zebrafish: Insight from a Comparative Gene Knockout Study.MARINE BIOTECHNOLOGY,7. |
| MLA | Liu, Chengjie,et al."Elovl2 But Not Elovl5 Is Essential for the Biosynthesis of Docosahexaenoic Acid (DHA) in Zebrafish: Insight from a Comparative Gene Knockout Study".MARINE BIOTECHNOLOGY (2020):7. |
入库方式： OAI收割
来源：水生生物研究所
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