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Assessing the intestinal bacterial community of farmed Nile tilapia (Oreochromis niloticus) by high-throughput absolute abundance quantification

文献类型：期刊论文

作者Wu, Zhenbing1,2; Wang, Shuyi1,2; Zhang, Qianqian1,3; Hao, Jingwen1,2; Lin, Yaoyao1,2; Zhang, Jinyong1,3; Li, Aihua1,3
刊名AQUACULTURE
出版日期2020-12-15
卷号529页码:9
ISSN号0044-8486
关键词Intestinal bacterial community Nile tilapia (Oreochromis niloticus) Internal standard strain Absolute abundance Quantitative bacterial ecology
DOI10.1016/j.aquaculture.2020.735688
通讯作者Li, Aihua(liaihua@ihb.ac.cn)
英文摘要Intestinal microbiota studies based on high-throughput sequencing have attracted increasing attention due to its vital impact on host health. However, the taxonomical profile in relative proportion does not reflect the quantity of the intestinal bacteria and the inter-sample differences among taxa. In this study, we applied a high-throughput absolute abundance quantification method that used an internal standard strain to simultaneously obtain both the relative and absolute abundances in the intestinal bacterial community of farmed Nile tilapia (Oreochromis niloticus). The results showed that the structure, relative and absolute abundances, and function of the intestinal indigenous microbiota didn't change significantly after adding a low concentration of internal standard strain, and a comprehensive quantitative intestinal bacterial ecology was obtained. We first found that the total absolute abundance of the intestinal indigenous bacteria in farmed Nile tilapia was 2.95 x 10(8) cells g(-1), and most genera concentrations were in the range of 10(3) to 10(5) cells g(-1). The intestinal bacterial community was dominated by Cetobacterium with a concentration of 10(8) cells g(-1), followed by Plesiomonas, Romboutsia, and Escherichia-Shigella with concentrations of over 10(7) cells g(-1). The potential intestinal pathogens Aeromonas and Streptococcus reached 10(5) cells g(-1) in the tilapia intestines, while the potential probiotics such as Lactobacillus and Enterococcus with a high concentration of over 10(6) cells g(-1) were detected. Furthermore, PICRUSt analysis revealed that functional genes involved in membrane transport, carbohydrate metabolism, and amino acid metabolism were enriched in the intestinal microbiota of tilapia. This study characterized the intestinal quantitative bacterial ecology of farmed Nile tilapia, providing foundational support for an in-depth understanding of the intestinal microbiota and its interaction mechanism with the host.
WOS关键词MICROBIAL COMMUNITIES ; GUT MICROBIOME ; WATER ; METABOLISM ; STANDARD ; REVEALS ; FISH ; CARP ; PCR
资助项目Key Project of Scientific & Technological Innovation of Hubei Province[2018ABA101] ; Wuhan Science and Technology Project[2019020701011480] ; FEBL project[2016FBZ04] ; Jiangsu Marine and Fisheries Bureau project[D2015-11] ; Jiangsu Marine and Fisheries Bureau project[D2017-3]
WOS研究方向Fisheries ; Marine & Freshwater Biology
语种英语
出版者ELSEVIER
WOS记录号WOS:000566469700016
资助机构Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project
源URL[http://ir.ihb.ac.cn/handle/342005/38622]  
专题水生生物研究所_水环境工程研究中心_期刊论文
通讯作者Li, Aihua
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Minist Agr, Key Lab Aquaculture Dis Control, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Zhenbing,Wang, Shuyi,Zhang, Qianqian,et al. Assessing the intestinal bacterial community of farmed Nile tilapia (Oreochromis niloticus) by high-throughput absolute abundance quantification[J]. AQUACULTURE,2020,529:9.
APA Wu, Zhenbing.,Wang, Shuyi.,Zhang, Qianqian.,Hao, Jingwen.,Lin, Yaoyao.,...&Li, Aihua.(2020).Assessing the intestinal bacterial community of farmed Nile tilapia (Oreochromis niloticus) by high-throughput absolute abundance quantification.AQUACULTURE,529,9.
MLA Wu, Zhenbing,et al."Assessing the intestinal bacterial community of farmed Nile tilapia (Oreochromis niloticus) by high-throughput absolute abundance quantification".AQUACULTURE 529(2020):9.

入库方式： OAI收割

来源：水生生物研究所

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