中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 水生生物研究所
  3. 中科院水生所知识产出（2009年前）
  4. 水生生物分子与细胞生物学研究中心
  5. 期刊论文
Expression of LamB Vaccine Antigen inWolffia globosa(Duck Weed) Against Fish Vibriosis

文献类型：期刊论文

作者Heenatigala, P. P. M.1,2,3; Sun, Zuoliang2,3; Yang, Jingjing2,3; Zhao, Xuyao2,3; Hou, Hongwei2,3
刊名FRONTIERS IN IMMUNOLOGY
出版日期2020-08-20
卷号11页码:9
关键词vibriosis Wolffia globosa LamB edible vaccine recombinant protein oral immunization
ISSN号1664-3224
DOI10.3389/fimmu.2020.01857
通讯作者Hou, Hongwei(houhw@ihb.ac.cn)
英文摘要Vibriosis is a commonly found bacterial disease identified among fish and shellfish cultured in saline waters. A multitude ofVibriospecies have been identified as the causative agents. LamB, a member of outer membrane protein (OMPs) family of these bacteria is conserved among allVibriospecies and has been identified as an efficient vaccine candidate against vibriosis. Rootless duckweed (Wolffia) is a tiny, edible aquatic plant possessing characteristics suitable for the utilization as a bioreactor. Thus, we attempted to express a protective edible vaccine antigen against fish vibriosis in nuclear-transformedWolffia. We amplifiedLamBgene from virulentVibrio alginolyticusand it was modified to maximize the protein expression level and translocate the protein to the endoplasmic reticulum (ER) in plants. It was cloned into binary vector pMYC under the control of CaMV 35S promoter and introduced intoWolffia globosabyAgrobacterium-mediated transformation. Integration and expression of theLamBgene was confirmed by genomic PCR and RT-PCR. Western blot analysis revealed accumulation of the LamB protein in 8 transgenic lines. The cross-protective property of transgenicWolffiawas evaluated by orally vaccinating zebrafish through feeding fresh transgenicWolffiaand subsequently challenging with virulentV. alginolyticus. High relative percent survival (RPS) of the vaccinated fish (63.3%) confirmed that fish immunized with transgenicWolffiawere well-protected fromVibrioinfection. These findings suggest thatWolffiaexpressed LamB could serve as an edible plant-based candidate vaccine model for fish vibriosis and feasibility of utilizingWolffiaas bioreactor to produce edible vaccines.
WOS关键词OUTER-MEMBRANE PROTEIN ; INFLUENZA-VIRUS H5N1 ; TRANSGENIC DUCKWEED ; PROTECTIVE ANTIGEN ; ORAL IMMUNIZATION ; M2E PEPTIDE ; IMMUNOGENICITY ; IDENTIFICATION ; GLYCOSYLATION ; INDUCTION
资助项目National Key RD Program[2018YFD0900801] ; Major Project of Technology Innovation Program of Hubei, China[2017ABA135] ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences[AB2018020] ; Hubei Province Postdoctoral Science Foundation
WOS研究方向Immunology
语种英语
WOS记录号WOS:000567886400001
出版者FRONTIERS MEDIA SA
资助机构National Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; National Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; National Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; National Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation
源URL[http://ir.ihb.ac.cn/handle/342005/38793]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
通讯作者Hou, Hongwei
作者单位1.Natl Aquat Resources Res & Dev Agcy NARA, Inland Aquat Resources & Aquaculture Div IARAD, Colombo, Sri Lanka
2.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Heenatigala, P. P. M.,Sun, Zuoliang,Yang, Jingjing,et al. Expression of LamB Vaccine Antigen inWolffia globosa(Duck Weed) Against Fish Vibriosis[J]. FRONTIERS IN IMMUNOLOGY,2020,11:9.
APA Heenatigala, P. P. M.,Sun, Zuoliang,Yang, Jingjing,Zhao, Xuyao,&Hou, Hongwei.(2020).Expression of LamB Vaccine Antigen inWolffia globosa(Duck Weed) Against Fish Vibriosis.FRONTIERS IN IMMUNOLOGY,11,9.
MLA Heenatigala, P. P. M.,et al."Expression of LamB Vaccine Antigen inWolffia globosa(Duck Weed) Against Fish Vibriosis".FRONTIERS IN IMMUNOLOGY 11(2020):9.

入库方式： OAI收割

来源：水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。