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  5. 期刊论文
Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species

文献类型：期刊论文

作者He, Xiaozhen7; Chen, Wenfeng7; Liu, Zhen6; Yu, Guirong7; Chen, Youbang7; Cai, Yi-Jun6; Sun, Ling7; Xu, Wanli7; Zhong, Lili7; Gao, Caixi7
刊名NUCLEIC ACIDS RESEARCH
出版日期2020-06-04
卷号48期号:10页码:19
ISSN号0305-1048
DOI10.1093/nar/gkaa195
通讯作者Sun, Qiang(qsun@ion.ac.cn) ; Yang, Yufeng(yangyufeng@outlook.com)
英文摘要Site-specific DNA double-strand breaks have been used to generate knock-in through the homology-dependent or -independent pathway. However, low efficiency and accompanying negative impacts such as undesirable indels or tumorigenic potential remain problematic. In this study, we present an enhanced reduced-risk genome editing strategy we named as NEO, which used either site-specific trans or cis double-nicking facilitated by four bacterial recombination factors (RecOFAR). In comparison to currently available approaches, NEO achieved higher knock-in (KI) germline transmission frequency (improving from zero to up to 10% efficiency with an average of 5-fold improvement for 8 loci) and 'cleaner' knock-in of long DNA fragments (up to 5.5 kb) into a variety of genome regions in zebrafish, mice and rats. Furthermore, NEO yielded up to 50% knock-in in monkey embryos and 20% relative integration efficiency in non-dividing primary human peripheral blood lymphocytes (hPBLCs). Remarkably, both on-target and off-target indels were effectively suppressed by NEO. NEO may also be used to introduce low-risk unrestricted point mutations effectively and precisely. Therefore, by balancing efficiency with safety and quality, the NEO method reported here shows substantial potential and improves the in vivo gene-editing strategies that have recently been developed.
WOS关键词HOMOLOGY-DIRECTED REPAIR ; DNA-REPAIR ; KNOCK-IN ; STRANDED-DNA ; CAS9 NICKASE ; BREAK REPAIR ; CRISPR-CAS9 ; ZEBRAFISH ; CRISPR/CAS9 ; PRECISE
资助项目Fujian Province Min-River Scholar Grant[XRC-0947] ; National Natural Science Foundation of China[31071296] ; National Natural Science Foundation of China[31771470] ; National Natural Science Foundation of China[31501083] ; National Natural Science Foundation of China[31970461] ; National Natural Science Foundation of China[31601894] ; National Natural Science Foundation of China[31270854] ; National Natural Science Foundation of China[91432111] ; Fuzhou University Sci-Tech Innovation Grant[XJJ1201] ; Fuzhou University Sci-Tech Innovation Grant[XJ1202] ; Shanghai Municipal Government Bureau of Science and Technology[18JC1410100] ; National Key Research and Development Program of China[2018YFC1003000] ; Startup Funds from Fuzhou University[XRC-1502] ; Fujian Natural Science Foundation[2017J0106] ; Fuzhou University[XRC-1463] ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant[JAT160071] ; National Basic Research Program of China[2011CBA00400] ; CAS Strategic Priority Research Program[XDB02050400]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000569071800003
出版者OXFORD UNIV PRESS
资助机构Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program
源URL[http://ir.ihb.ac.cn/handle/342005/38824]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
通讯作者Sun, Qiang; Yang, Yufeng
作者单位1.Fujian Med Univ, Union Hosp, Dept Gastr Surg, Fuzhou 350001, Fujian, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Tsinghua Univ, Zhejiang Prov Key Lab Appl Enzymol, Yangtze Delta Reg Inst, Jiaxing 314006, Zhejiang, Peoples R China
4.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China
5.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, State Key Lab Biomembrane & Membrane Engn, Beijing 100084, Peoples R China
6.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Inst Neurosci, State Key Lab Neurosci,CAS Key Lab Primate Neurob, Shanghai 200031, Peoples R China
7.Fuzhou Univ, Inst Life Sci, Fuzhou 350108, Fujian, Peoples R China
推荐引用方式
GB/T 7714		 He, Xiaozhen,Chen, Wenfeng,Liu, Zhen,et al. Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species[J]. NUCLEIC ACIDS RESEARCH,2020,48(10):19.
APA He, Xiaozhen.,Chen, Wenfeng.,Liu, Zhen.,Yu, Guirong.,Chen, Youbang.,...&Yang, Yufeng.(2020).Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species.NUCLEIC ACIDS RESEARCH,48(10),19.
MLA He, Xiaozhen,et al."Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species".NUCLEIC ACIDS RESEARCH 48.10(2020):19.

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来源：水生生物研究所

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