Molecular cloning, functional characterization and expression analysis of P65 subunit in response to GCRV infection in rare minnow (Gobiocypris rarus)
文献类型：期刊论文
| 作者 | Zhou, Man1,2; Wang, Bing1,2; Lin, Yusheng1,2; Zhang, Chuang3; Cao, Hong1,2 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2020-12-01 |
| 卷号 | 113页码:9 |
| ISSN号 | 0145-305X |
| 关键词 | P65 Innate immunity GCRV Antiviral Gobiocypris rarus |
| DOI | 10.1016/j.dci.2020.103786 |
| 通讯作者 | Cao, Hong(regancao@ihb.ac.cn) |
| 英文摘要 | P65, the all-important subunit of the transcription factor NF-kappa B, plays an important role in the regulation of immune response. In this study, the cDNA of P65 subunit of rare minnow Gobiocypris rarus (GrP65) was cloned, and its expression patterns and functional role in rare minnow were investigated. The GrP65cDNA encodes a polypeptide of 573 amino acids, containing a well-conserved Rel-homology domain (RHD). The amino acid sequence analysis showed that GrP65 shared 81% and 69% identity to the grass carp (Ctenopharyngodon idella) and human (Homo sapiens) orthologous, respectively. Phylogenetic analysis revealed that GrP65 clustered with homologues from other teleosts. Cellular distribution anallysis demonstrated that GrP65 was located in the cytoplasm and nucleus. Quantitative real-time PCR analysis showed that GrP65 was ubiquitously expressed in all examined tissues, but especially highly in liver. Temporal expression analysis in vivo showed that the expression levels of GrP65 were significantly up-regulated in liver in response to GCRV infection, which suggested that GrP65 might play a crucial role in recognition and responses to GCRV infection in fish. In addition, GrP65 activated several interferon (IFN) promoters and induced the expression of downstream IFN-stimulated genes (ISGs). Furthermore, overexpression of P65 remarkably decreased the GCRV proliferation, while knockdown of P65 obtained opposite effects. In summary, we systematically characterized GrP65 and demonstrated its role in the innate immune response to GCRV infections. |
| WOS关键词 | NF-KAPPA-B ; PATTERN-RECOGNITION RECEPTORS ; GRASS CARP ; TRANSCRIPTION ; INDUCTION ; PROTEIN ; ALPHA ; PHOSPHORYLATION ; COMPLEX ; RNA |
| 资助项目 | Grants of Chongqing Agricultural Investment Group Co., Ltd.[2017-4201-03-000047] |
| WOS研究方向 | Fisheries ; Immunology ; Zoology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000570248700008 |
| 资助机构 | Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. ; Grants of Chongqing Agricultural Investment Group Co., Ltd. |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/38831]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 通讯作者 | Cao, Hong |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Chongqing Fishery Sci Res Inst, Chongqing 400020, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Man,Wang, Bing,Lin, Yusheng,et al. Molecular cloning, functional characterization and expression analysis of P65 subunit in response to GCRV infection in rare minnow (Gobiocypris rarus)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,113:9. |
| APA | Zhou, Man,Wang, Bing,Lin, Yusheng,Zhang, Chuang,&Cao, Hong.(2020).Molecular cloning, functional characterization and expression analysis of P65 subunit in response to GCRV infection in rare minnow (Gobiocypris rarus).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,113,9. |
| MLA | Zhou, Man,et al."Molecular cloning, functional characterization and expression analysis of P65 subunit in response to GCRV infection in rare minnow (Gobiocypris rarus)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 113(2020):9. |
入库方式： OAI收割
来源：水生生物研究所
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