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Transcriptional Programs Underlying Cold Acclimation of Common Carp (Cyprinus carpioL.)

文献类型：期刊论文

作者Long, Yong2; Li, Xixi2,4,5; Li, Fengyang1,2; Ge, Guodong2,4,5; Liu, Ran1,2; Song, Guili2; Li, Qing2; Qiao, Zhigang3; Cui, Zongbin2,5
刊名FRONTIERS IN GENETICS
出版日期2020-09-23
卷号11页码:18
关键词cold acclimation common carp stress response transcriptional program gene expression genetic diversity alternative splicing
DOI10.3389/fgene.2020.556418
通讯作者Cui, Zongbin(zbcui@ihb.ac.cn)
英文摘要Properly regulated transcriptional responses to environmental perturbations are critical for the fitness of fish. Although gene expression profiles in the tissues of common carp upon cold stress were previously characterized, the transcriptional programs underlying cold acclimation are still not well known. In this study, the ability of three common carp strains including Hebao red carp (HB), Songpu mirror carp (SPM) and Yellow river carp (YR) to establish cold resistance after acclimation to a mild hypothermia stress at 18 degrees C for 24 h was confirmed by measurements of the critical thermal minimums (CTMin). The gene expression profiles of the brain and the heart from these strains under both control and cold-acclimated conditions were characterized with RNA-sequencing. The data of the three common carp strains with different genetic background were combined in the differential gene expression analyses to balance the effects of genetic diversity on gene expression. Marked effects of tissue origins on the cold-induced transcriptional responses were revealed by comparing the differentially expressed gene (DEG) lists of the two tissues. Functional categories including spliceosome and RNA splicing were highly enriched in the DEGs of both tissues. However, steroid biosynthesis was specifically enriched in DEGs of the brain and response to unfolded protein was solely enriched in DEGs of the heart. Consistent with the up-regulation of the genes involved in cholesterol biosynthesis, total cholesterol content of the brain was significantly increased upon cold stress. Moreover, cold-induced alternative splicing (AS) events were explored and AS of therbmx(RNA-binding motif protein, X chromosome) gene was confirmed by real-time quantitative PCR. Finally, a core set of cold responsive genes (CRGs) were defined by comparative transcriptomic analyses. Our data provide insights into the transcriptional programs underlying cold acclimation of common carp and offer valuable clues for further investigating the genetic determinants for cold resistance of farmed fish.
WOS关键词TEMPERATURE TOLERANCE ; GENE-EXPRESSION ; RNA LOCALIZATION ; CHOLESTEROL ; ANNOTATION ; RESISTANCE ; CYTOSCAPE ; RESPONSES ; PATHWAY ; BRAIN
资助项目National Key R&D Program of China[2018YFD0900302-2] ; National Natural Science Foundation of China[31772836] ; National Natural Science Foundation of China[31572610] ; National Natural Science Foundation of China[31721005] ; Youth Innovation Promotion Association of Chinese Academy of Sciences[2016305]
WOS研究方向Genetics & Heredity
语种英语
出版者FRONTIERS MEDIA SA
WOS记录号WOS:000575483000001
资助机构National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences
源URL[http://ir.ihb.ac.cn/handle/342005/38915]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
通讯作者Cui, Zongbin
作者单位1.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
3.Henan Normal Univ, Fisheries Coll, Xinxiang, Henan, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Guangdong Acad Sci, Guangdong Inst Microbiol, State Key Lab Appl Microbiol Southern China, Guangdong Prov Key Lab Microbial Culture Collect, Guangzhou, Peoples R China
推荐引用方式
GB/T 7714		 Long, Yong,Li, Xixi,Li, Fengyang,et al. Transcriptional Programs Underlying Cold Acclimation of Common Carp (Cyprinus carpioL.)[J]. FRONTIERS IN GENETICS,2020,11:18.
APA Long, Yong.,Li, Xixi.,Li, Fengyang.,Ge, Guodong.,Liu, Ran.,...&Cui, Zongbin.(2020).Transcriptional Programs Underlying Cold Acclimation of Common Carp (Cyprinus carpioL.).FRONTIERS IN GENETICS,11,18.
MLA Long, Yong,et al."Transcriptional Programs Underlying Cold Acclimation of Common Carp (Cyprinus carpioL.)".FRONTIERS IN GENETICS 11(2020):18.

入库方式： OAI收割

来源：水生生物研究所

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