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Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803

文献类型：期刊论文

作者Wu, Dongqing1,2,3; Wang, Yali2,3; Xu, Xudong2,3
刊名FRONTIERS IN MICROBIOLOGY
出版日期2020-06-09
卷号11页码:8
关键词Synechocystis type I RM system gene regulation glgP glycogen catabolism
ISSN号1664-302X
DOI10.3389/fmicb.2020.01258
通讯作者Xu, Xudong(xux@ihb.ac.cn)
英文摘要Increasing evidence has shown that DNA methylation is involved in gene regulation in prokaryotes. However, there have been very limited reports about the role of DNA methylation in regulation of gene expression and physiological functions in cyanobacteria. InSynechocystissp. PCC 6803, four genes on the plasmid pSYSX are predicted to encode the type I restriction-methylation system,slr6095andslr6096for the M subunit,slr6097for the S subunit andslr6102for theRsubunit. Compared to the wild type,slr6095,slr6096, andslr6097mutants lacked the GG(m6)AN(7)TTGG/CCA(m6)AN(7)TCC methylation in genomic DNA. Transcriptomic analysis indicated that 171 genes were reproducibly up- or down-regulated in all three mutants relative to the wild type. The changed expression of some genes, includingsll1356for glycogen phosphorylase (GlgP), was associated with the loss of GG(m6)AN(7)TTGG/CCA(m6)AN(7)TCC methylation in the coding regions or the upstream non-coding sequences. Inactivation ofslr6095,slr6096, orslr6097increased the expression ofsll1356and the GlgP activity but lowered the glycogen content. These results indicated that the DNA methylation by a type I RM system could alter the expression of certain genes and physiological functions inSynechocystissp. PCC 6803.
WOS关键词DNA METHYLTRANSFERASES ; BACTERIAL GLYCOGEN ; SYNECHOCYSTIS ; TOLERANCE ; STRESS
资助项目National Natural Science Foundation of China[31470203] ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS[2019FBZ09] ; Knowledge Innovation Project of Hubei Province[2017CFA021]
WOS研究方向Microbiology
语种英语
WOS记录号WOS:000543513600001
出版者FRONTIERS MEDIA SA
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province
源URL[http://ir.ihb.ac.cn/handle/342005/38372]  
专题水生生物研究所_藻类生物学及应用研究中心_期刊论文
通讯作者Xu, Xudong
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Dongqing,Wang, Yali,Xu, Xudong. Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803[J]. FRONTIERS IN MICROBIOLOGY,2020,11:8.
APA Wu, Dongqing,Wang, Yali,&Xu, Xudong.(2020).Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803.FRONTIERS IN MICROBIOLOGY,11,8.
MLA Wu, Dongqing,et al."Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803".FRONTIERS IN MICROBIOLOGY 11(2020):8.

入库方式： OAI收割

来源：水生生物研究所

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