中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 水生生物研究所
  3. 中科院水生所知识产出（2009年前）
  4. 藻类生物学及应用研究中心
  5. 期刊论文
Functional Dissection of Genes Encoding DNA Polymerases Based on Conditional Mutants in the Heterocyst-Forming CyanobacteriumAnabaenaPCC 7120

文献类型：期刊论文

作者Xing, Wei-Yue1,2,3; Xie, Li-rui1,2; Zeng, Xiaoli1,2; Yang, Yiling1,2; Zhang, Cheng-Cai1,2,4,5
刊名FRONTIERS IN MICROBIOLOGY
出版日期2020-06-03
卷号11页码:13
ISSN号1664-302X
关键词heterocyst DNA replication cell cycle SOS response cyanobacteria
DOI10.3389/fmicb.2020.01108
通讯作者Yang, Yiling(yangyiling@ihb.ac.cn) ; Zhang, Cheng-Cai(cczhang@ihb.ac.cn)
英文摘要The filamentous cyanobacteriumAnabaenasp. PCC 7120 develops N-2-fixing heterocyst cells under condition of combined-nitrogen deprivation and constitutes an excellent model for studying cell differentiation. The mechanism of heterocyst development has been extensively investigated and a network of regulating factors has been identified. A few studies have showed that the process of heterocyst differentiation relates with cell cycle events, but further investigation is still required to understand this relationship. In a previous study, we created a conditional mutant of PolI encoding gene,polA, by using a CRISPR/Cpf1 gene-editing technique. Here, we were able to create another conditional mutant of a PolIII encoding genednaENIusing a similar strategy and subsequently confirmed the essential roles of bothpolAanddnaENIin DNA replication. Further investigation on the phenotype of the mutants showed that lack of PolI caused defects in chromosome segregation and cell division, while lack of DnaENI (PolIII) prevented bulk DNA synthesis, causing significant loss of DNA content. Our findings also suggested the possible existence of a SOS-response like mechanism operating inAnabaenaPCC 7120. Moreover, we found that heterocyst development was differently affected in the two conditional mutants, with double heterocysts/proheterocysts found in PolI conditional mutant. We further showed that formation of such double heterocysts/proheterocysts are likely caused by the difficulty in nucleoids segregation, resulting delayed, or non-complete closure of the septum between the two daughter cells. This study uncovers a link between DNA replication process and heterocyst differentiation, paving the way for further studies on the relationship between cell cycle and cell development.
WOS关键词NITROGEN-FIXING CYANOBACTERIUM ; PATTERN-FORMATION ; ESCHERICHIA-COLI ; ANABAENA ; DIFFERENTIATION ; LEXA ; RECA ; MUTAGENESIS ; EXPRESSION ; PROTEINS
资助项目Key Research Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDJ-SSW-SMC016] ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences
WOS研究方向Microbiology
语种英语
出版者FRONTIERS MEDIA SA
WOS记录号WOS:000543342600001
资助机构Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences
源URL[http://ir.ihb.ac.cn/handle/342005/38381]  
专题水生生物研究所_藻类生物学及应用研究中心_期刊论文
通讯作者Yang, Yiling; Zhang, Cheng-Cai
作者单位1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China
2.Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Aix Marseille Univ, Inst WUT AMU, Marseille, France
5.Wuhan Univ Technol, Wuhan, Peoples R China
推荐引用方式
GB/T 7714		 Xing, Wei-Yue,Xie, Li-rui,Zeng, Xiaoli,et al. Functional Dissection of Genes Encoding DNA Polymerases Based on Conditional Mutants in the Heterocyst-Forming CyanobacteriumAnabaenaPCC 7120[J]. FRONTIERS IN MICROBIOLOGY,2020,11:13.
APA Xing, Wei-Yue,Xie, Li-rui,Zeng, Xiaoli,Yang, Yiling,&Zhang, Cheng-Cai.(2020).Functional Dissection of Genes Encoding DNA Polymerases Based on Conditional Mutants in the Heterocyst-Forming CyanobacteriumAnabaenaPCC 7120.FRONTIERS IN MICROBIOLOGY,11,13.
MLA Xing, Wei-Yue,et al."Functional Dissection of Genes Encoding DNA Polymerases Based on Conditional Mutants in the Heterocyst-Forming CyanobacteriumAnabaenaPCC 7120".FRONTIERS IN MICROBIOLOGY 11(2020):13.

入库方式： OAI收割

来源：水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。