The Proposed Neurotoxin beta-N-Methylamino-l-Alanine (BMAA) Is Taken up through Amino-Acid Transport Systems in the CyanobacteriumAnabaenaPCC 7120
文献类型:期刊论文
作者 | Wang, Zi-Qian2,3,4; Wang, Suqin2,3,4; Zhang, Ju-Yuan2,3; Lin, Gui-Ming2,3; Gan, Nanqin2,3; Song, Lirong2,3; Zeng, Xiaoli2,3; Zhang, Cheng-Cai1,2,3,5,6 |
刊名 | TOXINS |
出版日期 | 2020-08-01 |
卷号 | 12期号:8页码:14 |
关键词 | neurodegenerative disease cyanobacteria amino acids transport BMAA resistance |
DOI | 10.3390/toxins12080518 |
通讯作者 | Zeng, Xiaoli(zengxl@ihb.ac.cn) ; Zhang, Cheng-Cai(cczhang@ihb.ac.cn) |
英文摘要 | Produced by cyanobacteria and some plants, BMAA is considered as an important environmental factor in the occurrence of some neurodegenerative diseases. Neither the underlying mechanism of its toxicity, nor its biosynthetic or metabolic pathway in cyanobacteria is understood. Interestingly, BMAA is found to be toxic to some cyanobacteria, making it possible to dissect the mechanism of BMAA metabolism by genetic approaches using these organisms. In this study, we used the cyanobacteriumAnabaenaPCC 7120 to isolate BMAA-resistant mutants. Following genomic sequencing, several mutations were mapped to two genes involved in amino acids transport, suggesting that BMAA was taken up through amino acid transporters. This conclusion was supported by the protective effect of several amino acids against BMAA toxicity. Furthermore, targeted inactivation of genes encoding different amino acid transport pathways conferred various levels of resistance to BMAA. One mutant inactivating all three major amino acid transport systems could no longer take up BMAA and gained full resistance to BMAA toxicity. Therefore, BMAA is a substrate of amino acid transporters, and cyanobacteria are interesting models for genetic analysis of BMAA transport and metabolism. |
WOS关键词 | 2,4-DIAMINOBUTYRIC ACID ; CYANOBACTERIAL NEUROTOXINS ; FRESH-WATER ; DAB ; GROWTH ; SEEDS |
资助项目 | Key Research Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDJ-SSW-SMC016] ; Featured Institute Service Projects from the Institute of Hydrobiology ; Chinese Academy of Sciences[Y85Z061601] ; Chinese Academy of Sciences[Y65Z021501] |
WOS研究方向 | Food Science & Technology ; Toxicology |
语种 | 英语 |
出版者 | MDPI |
WOS记录号 | WOS:000564125300001 |
资助机构 | Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Featured Institute Service Projects from the Institute of Hydrobiology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences |
源URL | [http://ir.ihb.ac.cn/handle/342005/38717] |
专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
通讯作者 | Zeng, Xiaoli; Zhang, Cheng-Cai |
作者单位 | 1.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Aix Marseille Univ, Inst AMU WUT, Wuhan 430072, Peoples R China 6.Wuhan Univ Technol, Wuhan 430072, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Zi-Qian,Wang, Suqin,Zhang, Ju-Yuan,et al. The Proposed Neurotoxin beta-N-Methylamino-l-Alanine (BMAA) Is Taken up through Amino-Acid Transport Systems in the CyanobacteriumAnabaenaPCC 7120[J]. TOXINS,2020,12(8):14. |
APA | Wang, Zi-Qian.,Wang, Suqin.,Zhang, Ju-Yuan.,Lin, Gui-Ming.,Gan, Nanqin.,...&Zhang, Cheng-Cai.(2020).The Proposed Neurotoxin beta-N-Methylamino-l-Alanine (BMAA) Is Taken up through Amino-Acid Transport Systems in the CyanobacteriumAnabaenaPCC 7120.TOXINS,12(8),14. |
MLA | Wang, Zi-Qian,et al."The Proposed Neurotoxin beta-N-Methylamino-l-Alanine (BMAA) Is Taken up through Amino-Acid Transport Systems in the CyanobacteriumAnabaenaPCC 7120".TOXINS 12.8(2020):14. |
入库方式: OAI收割
来源:水生生物研究所
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