中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 水生生物研究所
  3. 中科院水生所知识产出（2009年前）
  4. 藻类生物学及应用研究中心
  5. 期刊论文
Automated accelerated solvent extraction method for total lipid analysis of microalgae

文献类型：期刊论文

作者Chen, Wei1,2; Liu, Yongmei2; Song, Lirong4; Sommerfeld, Milton1; Hu, Qiang1,3
刊名ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
出版日期2020-10-01
卷号51页码:9
关键词Total lipids Comparison studies Microalgae Accelerated solvent extraction Conventional methods Biofuel Lipid quality
ISSN号2211-9264
DOI10.1016/j.algal.2020.102080
通讯作者Chen, Wei(chenwei@qau.edu.cn) ; Hu, Qiang(huqiang@asu.edu)
英文摘要For algae-based biofuel researches, it is usually necessary to extract and quantitatively measure total lipids. The most popular total lipid determination methods for tissues are solvent-based methods with either manual or Soxhlet extraction. Although some investigators have modified these traditional methods, the modifications have rarely been specified in detail, nor has their effectiveness been critically evaluated on microalgae biomass. As a result, there are often significant differences in the analyses of total lipid contents among investigators and laboratories. To assess the effectiveness of different total lipid extraction protocols for microalgae, several conventional lipid extraction methods were systematically compared with an accelerated solvent extraction (ASE) method. Optimal conditions for the ASE method were determined to be three cycles of 3 min at 125 degrees C using a 5 ml extraction cell containing 20 to 150 mg of dry algal biomass. Critical comparisons and assessments of these total lipid extraction methods from microalgae were performed for both total lipid extraction efficiency and extracted lipid quality (lipid decomposition and oxidation). Results obtained from five algal species indicated that hot Soxhlet extraction and ASE were comparable in total lipid extraction efficiencies, which were higher than the efficiencies obtained from conventional manual extraction methods. However, lipid oxidation resulting from different extraction methods indicated that continuous heating with a hot Soxhlet extraction method could lead to lipid oxidation. Considering the efficiency and quality of the extracted lipids, extraction time, extraction steps and consumed solvent volumes, ASE is a very promising method for total lipid extraction and analysis of lipids from microalgal biomass.
WOS关键词FATTY-ACID-COMPOSITION ; ALGAE ; SOXHLET ; YEAST ; BLIGH ; DYER
资助项目National Natural Science Foundation of China[NSFC 41276150]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000576964300015
出版者ELSEVIER
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.ihb.ac.cn/handle/342005/38952]  
专题水生生物研究所_藻类生物学及应用研究中心_期刊论文
通讯作者Chen, Wei; Hu, Qiang
作者单位1.Arizona State Univ, Dept Appl Sci & Math, 7001 E Williams Field Rd, Mesa, AZ 85212 USA
2.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266237, Peoples R China
3.Shenzhen Univ, Inst Adv Study, Shenzhen 518060, Guangdong, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Wei,Liu, Yongmei,Song, Lirong,et al. Automated accelerated solvent extraction method for total lipid analysis of microalgae[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2020,51:9.
APA Chen, Wei,Liu, Yongmei,Song, Lirong,Sommerfeld, Milton,&Hu, Qiang.(2020).Automated accelerated solvent extraction method for total lipid analysis of microalgae.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,51,9.
MLA Chen, Wei,et al."Automated accelerated solvent extraction method for total lipid analysis of microalgae".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 51(2020):9.

入库方式： OAI收割

来源：水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。