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Production of Highly Active Extracellular Amylase and Cellulase FromBacillus subtilisZIM3 and a Recombinant Strain With a Potential Application in Tobacco Fermentation

文献类型：期刊论文

作者Dai, Jingcheng5; Dong, Aijun4; Xiong, Guoxi4; Liu, Yaqi2,5; Hossain, Md Shahdat1,3,5; Liu, Shuangyuan3,5; Gao, Na3,5; Li, Shuyang3,5; Wang, Jing3,5; Qiu, Dongru3,5
刊名FRONTIERS IN MICROBIOLOGY
出版日期2020-07-21
卷号11页码:14
关键词amylase cellulase heterogeneous expression bacillus tobacco
ISSN号1664-302X
DOI10.3389/fmicb.2020.01539
通讯作者Dong, Aijun(11013213@hbtobacco.cn) ; Qiu, Dongru(qiu@ihb.ac.cn)
英文摘要In this study, a series of bacteria capable of degrading starch and cellulose were isolated from the aging flue-cured tobacco leaves. Remarkably, there was a thermophilic bacterium,Bacillus subtilisZIM3, that can simultaneously degrade both starch and cellulose at a wide range of temperature and pH values. Genome sequencing, comparative genomics analyses, and enzymatic activity assays showed that the ZIM3 strain expressed a variety of highly active plant biomass-degrading enzymes, such as the amylase AmyE1 and cellulase CelE1. Thein vitroand PhoA-fusion assays indicated that these enzymes degrading complex plant biomass into fermentable sugars were secreted into ambient environment to function. Besides, the amylase and cellulase activities were further increased by three- to five-folds by using overexpression. Furthermore, a fermentation strategy was developed and the biodegradation efficiency of the starch and cellulose in the tobacco leaves were improved by 30-48%. These results reveal thatB. subtilisZIM3 and the recombinant strain exhibited high amylase and cellulase activities for efficient biodegradation of starch and cellulose in tobacco and could potentially be applied for industrial tobacco fermentation.
WOS关键词THERMOSTABLE ALPHA-AMYLASE ; BACILLUS ; INOCULATION ; REAGENT ; LEAVES ; GENES ; WASTE
资助项目National Natural Science Foundation of China[91751106] ; Key Research Program of the Chinese Academy of Sciences[XDA23040401] ; Key Research Program of the Chinese Academy of Sciences[Y15103-1-401] ; Enterprise Principal Project[Y84108]
WOS研究方向Microbiology
语种英语
WOS记录号WOS:000558895600001
出版者FRONTIERS MEDIA SA
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project
源URL[http://ir.ihb.ac.cn/handle/342005/38687]  
专题水生生物研究所_其他_期刊论文
通讯作者Dong, Aijun; Qiu, Dongru
作者单位1.Natl Inst Biotechnol, Dhaka, Bangladesh
2.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.China Tobacco Hubei Ind Co Ltd, Technol Res Ctr, Wuhan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
推荐引用方式
GB/T 7714		 Dai, Jingcheng,Dong, Aijun,Xiong, Guoxi,et al. Production of Highly Active Extracellular Amylase and Cellulase FromBacillus subtilisZIM3 and a Recombinant Strain With a Potential Application in Tobacco Fermentation[J]. FRONTIERS IN MICROBIOLOGY,2020,11:14.
APA Dai, Jingcheng.,Dong, Aijun.,Xiong, Guoxi.,Liu, Yaqi.,Hossain, Md Shahdat.,...&Qiu, Dongru.(2020).Production of Highly Active Extracellular Amylase and Cellulase FromBacillus subtilisZIM3 and a Recombinant Strain With a Potential Application in Tobacco Fermentation.FRONTIERS IN MICROBIOLOGY,11,14.
MLA Dai, Jingcheng,et al."Production of Highly Active Extracellular Amylase and Cellulase FromBacillus subtilisZIM3 and a Recombinant Strain With a Potential Application in Tobacco Fermentation".FRONTIERS IN MICROBIOLOGY 11(2020):14.

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来源：水生生物研究所

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