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Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China

文献类型：期刊论文

作者Wang, Ping1,2; Jie, Li3; He, Tian Tian1; Li, Nan1; Mo, Zhao Lan3; Nie, Pin1,2,4; Xie, Hai Xia1
刊名JOURNAL OF FISH DISEASES
出版日期2020-07-27
页码10
关键词Aeromonas salmonicidasubsp masoucida apoptosis identification invasion virulence
ISSN号0140-7775
DOI10.1111/jfd.13224
通讯作者Nie, Pin(pinnie@ihb.ac.cn) ; Xie, Hai Xia(xieh@ihb.ac.cn)
英文摘要Aeromonas salmonicidais a gram-negative bacterium that is the causative agent of furunculosis. AnA. salmonicidastrain was isolated from diseased turbot (Scophthalmus maximus) with the sign of furunculosis from North China. Based onvapAgene, the strain was further classified asA. salmonicidasubsp.masoucidaRZ6S-1. Culturing RZ6S-1 strain at high temperature (28 degrees C) obtained the virulence attenuated strain RZ6S. Genome sequence comparison between the two strains revealed the loss of the type IV secretion system (T4SS) and type III secretion system (T3SS) from the native plasmid pAsmB-1 and pAsmC-1 of wild-type strain RZ6S-1, respectively. Further study demonstrated that the wild-type strain RZ6S-1, but not its derivative mutant RZ6S, can stimulate apoptosis. Elevated protein level of cleaved caspase-3 was detected from epithelioma papulosum cyprinid (EPC) cells infected with wild-type strain RZ6S-1 as compared with that infected with RZ6S strain. Meanwhile, the invasion of the mutant strain RZ6S was about 17-fold higher than the wild-type strain RZ6S-1, suggesting that some protein(s) fromA. salmonicidasubsp.masoucidaRZ6S-1 suppress its invasion. The RZ6S mutant strain was attenuated, since its LD(50)is over 10,000 times higher compared to the wild-type strain as revealed in the turbot infection model.
WOS关键词ATYPICAL AEROMONAS-SALMONICIDA ; III SECRETION SYSTEM ; SUBSP-SALMONICIDA ; EFFECTOR PROTEIN ; VIRULENCE ; INFECTION ; GENES
资助项目National Natural Science Foundation of China[U1706205] ; Agriculture Research System of China[CARS-46]
WOS研究方向Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000552556400001
出版者WILEY
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China
源URL[http://ir.ihb.ac.cn/handle/342005/36882]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Nie, Pin; Xie, Hai Xia
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao, Peoples R China
3.Chinese Acad Fishery Sci, Key Lab Maricultural Organism Dis Control, Minist Agr & Rural Affairs, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Ping,Jie, Li,He, Tian Tian,et al. Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China[J]. JOURNAL OF FISH DISEASES,2020:10.
APA Wang, Ping.,Jie, Li.,He, Tian Tian.,Li, Nan.,Mo, Zhao Lan.,...&Xie, Hai Xia.(2020).Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China.JOURNAL OF FISH DISEASES,10.
MLA Wang, Ping,et al."Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China".JOURNAL OF FISH DISEASES (2020):10.

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来源：水生生物研究所

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