SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY
文献类型:期刊论文
作者 | Zhang Yongan2; Nie Pin2 |
刊名 | 中国海洋湖沼学报:英文版
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出版日期 | 2002 |
卷号 | 020期号:004页码:332 |
关键词 | 免疫球蛋白 中国金鲈 多克隆抗体 体液免疫反应 鱼类 |
ISSN号 | 0254-4059 |
英文摘要 | Serum immunoglobulin from the mandarin fish,or the so-called Chinese perch,Siniperca chuatsi(Basilewsky),was successfully purified using affinity chromatography.Heavy and light chains were detected on electrophoresis gel,with molecular weights being estimated at 72 and 29 kDa,respectively.The tetrameric IgM of S.chuatsi was calculated to be 808 kDa.The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Westem blot analysis.The antisera reacted strongly with the heavy chains of S.chuatsi immunoglobulin.Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody. |
语种 | 英语 |
源URL | [http://ir.ihb.ac.cn/handle/342005/37835] ![]() |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
作者单位 | 1.Institute of Hydrobiology, Chinese Academy of Sciences 2.Institute of Hydrobiology, Chinese Academy of Sciences |
推荐引用方式 GB/T 7714 | Zhang Yongan,Nie Pin. SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY[J]. 中国海洋湖沼学报:英文版,2002,020(004):332. |
APA | Zhang Yongan,&Nie Pin.(2002).SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY.中国海洋湖沼学报:英文版,020(004),332. |
MLA | Zhang Yongan,et al."SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY".中国海洋湖沼学报:英文版 020.004(2002):332. |
入库方式: OAI收割
来源:水生生物研究所
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