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A rapid method of sex-specific marker discovery based on NGS and determination of the XX / XY sex-determination system in Channa maculata

文献类型：期刊论文

作者Yang, Cheng1,2; Huang, Rong2; Ou, Mi3; Gui, Bin2; Zhao, Jian3; He, Libo2; Li, Yongming2; Liao, Lanjie2; Chen, Kunci3; Wang, Yaping2,4
刊名AQUACULTURE
出版日期2020-11-15
卷号528页码:6
关键词Sex-specific marker Next-generation sequencing (NGS) Sex-determination mechanism Unisexual breeding
ISSN号0044-8486
DOI10.1016/j.aquaculture.2020.735499
通讯作者Huang, Rong(huangrong@ihb.ac.cn) ; Wang, Yaping(wangyp.ihb@gmail.com)
英文摘要The discovery of sex-specific markers is of great significance for the study of unisexual breeding and sex-determination mechanisms of fish. The all-male hybrid snakehead is a freshwater aquaculture species with high economic value. The breeding of all-male hybrid snakeheads depends on the discovery of sex-specific markers of Taiwan snakeheads. In this study, 24 females and 24 males of Taiwan snakeheads were sequenced by NGS, and then a set of biological information analysis process was developed to speculate that Taiwan snakeheads were XX / XY sex-determining system, and 1250 male-related contigs were assembled; In combination with indels and SNP sites analysis, Chelex 100 DNA extraction and an HRM scan, two molecular markers (CM286 and CM1053) were selected from 29 male-related contigs, which could clearly distinguish XX females, XY males, and YY supermales; Then, it was confirmed by Sanger sequencing that Taiwan snakeheads had a male heterogamete type, and the ratio of females to males in three full-sib families was all close to 1:1 (P >.05). Therefore, Taiwan snakeheads were determined to be the XX / XY sex-determining system. The results showed that the effective sex-specific markers, combined with the Chelex 100 DNA extraction and HRM scan technology, could realize high-throughput and rapid detection of the three sexes (XX, XY, and YY) of Taiwan snakeheads, and could be directly applied to the breeding of the all-male hybrid snakehead. This process of bioinformatic analysis for sexspecific marker discovery is simple and efficient, and can also be applied to the study of other fish sex-specific marker discovery and sex-determination mechanisms.
WOS关键词PROVIDES INSIGHTS ; IDENTIFICATION ; GENOME ; DIFFERENTIATION ; DIVERSITY ; EVOLUTION ; CATFISH ; FISH
资助项目National Key Research & Development Program of China[2018YFD0901201] ; National Natural Science Foundation of China[31672647]
WOS研究方向Fisheries ; Marine & Freshwater Biology
语种英语
WOS记录号WOS:000553693000007
出版者ELSEVIER
资助机构National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.ihb.ac.cn/handle/342005/38585]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Huang, Rong; Wang, Yaping
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr, Guangzhou 510380, Peoples R China
4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Cheng,Huang, Rong,Ou, Mi,et al. A rapid method of sex-specific marker discovery based on NGS and determination of the XX / XY sex-determination system in Channa maculata[J]. AQUACULTURE,2020,528:6.
APA Yang, Cheng.,Huang, Rong.,Ou, Mi.,Gui, Bin.,Zhao, Jian.,...&Wang, Yaping.(2020).A rapid method of sex-specific marker discovery based on NGS and determination of the XX / XY sex-determination system in Channa maculata.AQUACULTURE,528,6.
MLA Yang, Cheng,et al."A rapid method of sex-specific marker discovery based on NGS and determination of the XX / XY sex-determination system in Channa maculata".AQUACULTURE 528(2020):6.

入库方式： OAI收割

来源：水生生物研究所

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