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Identifying difference in primordial germ cells between XX female and XY male yellow catfish embryos

文献类型：期刊论文

作者Ge, Si2; Dan, Cheng1; Xiong, Yang2; Gong, Gaorui2; Mei, Jie2; Guo, Wenjie2; Li, Xiaohui2
刊名GENE
出版日期2020-11-30
卷号761页码:7
关键词Primordial germ cell Yellow catfish (Pelteobagrus fulvidraco) 3 ' untranslated region Nanos homolog 1-like Dead end Vasa
ISSN号0378-1119
DOI10.1016/j.gene.2020.145037
通讯作者Guo, Wenjie(guowenjie@mail.hzau.edu.cn) ; Li, Xiaohui(lixiaohui@mail.hzau.edu.cn)
英文摘要Primordial germ cells (PGCs) are singled out from somatic cells very early during embryogenesis, then they migrate towards the genital ridge and differentiate into gametes through oogenesis or spermatogenesis. Labeling PGCs with Localized RNA expression (LRE) technique by fluorescent proteins has been widely applied among teleost species to study the germ cell development and gonad differentiation. In this study, we first cloned and characterized the 3' untranslated regions (3'UTRs) of nanos homolog 1-like (nos1l), dead end (dnd), and vasa in yellow catfish (Pelteobagrus fulvidraco), and then synthesized the GFP-nosll/dnd/vasa 3'UTR mRNAs. Each of these three 3'UTRs could label PGCs in yellow catfish embryos, of which, vasa 3'UTR exhibited the highest labeling efficiency. To identify the differences in PGCs at embryonic stage, XX all-female and XY all-male yellow catfish embryos were produced and injected with GFP-vasa 3'UTR mRNA. We observed the PGC migration route in these two monosex embryos from 24 hpf to 7 dpf, and found there was no difference between them. Besides, the PGC number was counted at 48 hpf, and the result showed that the average PGC number in XX females (11.3) was significantly larger than that in XY males (8.1). These findings provide an insight into the development of PGCs in yellow catfish embryos and the relationship between embryonic PGC number and the later gonad differentiation.
WOS关键词GREEN FLUORESCENT PROTEIN ; SEX-DIFFERENTIATION ; EXPRESSION ; MEDAKA ; GENE ; DIMORPHISM ; VISUALIZATION ; MARKERS
资助项目National Natural Science Foundation of China[31900585] ; Fundamental Research Funds for the Central Universities[2662018QD051]
WOS研究方向Genetics & Heredity
语种英语
WOS记录号WOS:000569085200011
出版者ELSEVIER
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
源URL[http://ir.ihb.ac.cn/handle/342005/38821]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Guo, Wenjie; Li, Xiaohui
作者单位1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
推荐引用方式
GB/T 7714		 Ge, Si,Dan, Cheng,Xiong, Yang,et al. Identifying difference in primordial germ cells between XX female and XY male yellow catfish embryos[J]. GENE,2020,761:7.
APA Ge, Si.,Dan, Cheng.,Xiong, Yang.,Gong, Gaorui.,Mei, Jie.,...&Li, Xiaohui.(2020).Identifying difference in primordial germ cells between XX female and XY male yellow catfish embryos.GENE,761,7.
MLA Ge, Si,et al."Identifying difference in primordial germ cells between XX female and XY male yellow catfish embryos".GENE 761(2020):7.

入库方式： OAI收割

来源：水生生物研究所

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