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Molecular identification and function characterization of four finTRIM genes from the immortal fish cell line, EPC

文献类型：期刊论文

作者Li, Yi-Lin1,4; Zhao, Xiang1,4; Gong, Xiu-Ying1,4; Dan, Cheng1,4; Gui, Jian-Fang1,3,4; Zhang, Yi-Bing1,2,3,4
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期2020-12-01
卷号113页码:11
ISSN号0145-305X
关键词finTRIM ISGs Negative regulation IFN antiviral Response
DOI10.1016/j.dci.2020.103775
通讯作者Zhang, Yi-Bing(ybzhang@ihb.ac.cn)
英文摘要In mammals, tripartite motif (TRIM)-containing proteins are involved in interferon (IFN)-mediated antiviral response as pivotal players endowed with antiviral effects and modulatory capacity. Teleost fish have a unique subfamily of TRIM, called finTRIM (fish novel TRIM, FTR) generated by genus- or species-specific duplication of TRIM genes. Herein, four TRIM genes are identified from Epithelioma papulosum cyprini (EPC) cells, and phylogenetically close to the members of finTRIM, thus named FTREPC1, FTREPC2, FTREPC3 and FTREPC4. Despite high similarity in nucleotide sequence, FTREPC1/2 genes encode two proteins with a typically consecutive tripartite motif followed by a C-terminal B30.2 domain, while FTREPC3/4-encoding proteins retain only a RING domain due to early termination of translation. They are induced by poly(I:C), GCRV and SVCV as IFN-stimulated genes (ISGs), and this induction is severely impaired by blockade of STAT1 pathway and is dependent on a typical ISRE motif within the 5' untranslated regions (5'UTRs) of FTREPC1/2/3/4 genes. Whereas overexpression of FTREPC1/2/3/4 alone does not activate fish IFN promoters, overexpression of FTREPC1 or FTREPC2, rather than FTREPC3 and FTREPC4, significantly impairs intracellular poly(I:C)-triggered activation of fish IFN promoters. Consistently, FTREPC1/2 promote virus replication through negatively regulating IFN response. Our results provide evidence for the involvement of EPC finTRIM proteins in IFN antiviral response and insights into genus- or species-specific regulation of fish innate immune pathways.
WOS关键词ANTIVIRAL IMMUNE-RESPONSE ; NEGATIVE REGULATION ; FAMILY ; EXERTS ; PROTEIN ; UBIQUITINATION ; IRIDOVIRUS ; PATHWAY ; TRIM13 ; ROLES
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24010308] ; National Key R&D Program of China[2018YFD0900302] ; National Natural Science Foundation[31772875] ; National Natural Science Foundation[31972826] ; Freshwater Ecology and Biotechnology Laboratory[2019FBZ04]
WOS研究方向Fisheries ; Immunology ; Zoology
语种英语
出版者ELSEVIER SCI LTD
WOS记录号WOS:000571143400002
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory
源URL[http://ir.ihb.ac.cn/handle/342005/38862]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Zhang, Yi-Bing
作者单位1.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Innovat Acad Seed Design, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714		 Li, Yi-Lin,Zhao, Xiang,Gong, Xiu-Ying,et al. Molecular identification and function characterization of four finTRIM genes from the immortal fish cell line, EPC[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,113:11.
APA Li, Yi-Lin,Zhao, Xiang,Gong, Xiu-Ying,Dan, Cheng,Gui, Jian-Fang,&Zhang, Yi-Bing.(2020).Molecular identification and function characterization of four finTRIM genes from the immortal fish cell line, EPC.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,113,11.
MLA Li, Yi-Lin,et al."Molecular identification and function characterization of four finTRIM genes from the immortal fish cell line, EPC".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 113(2020):11.

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来源：水生生物研究所

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