中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 水生生物研究所
  3. 鱼类生物学及渔业生物技术研究中心
  4. 期刊论文
Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila

文献类型：期刊论文

作者Xiong, Fan4; Xiong, Jing1; Wu, Ya Fang1; Cao, Lu3,4; Huang, Wen Shu1; Chang, Ming Xian2,3,4
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2020-10-01
卷号105页码:297-309
ISSN号1050-4648
关键词Transcriptome analysis European eel Aeromonas hydrophila Cytosolic DNA-Sensing pathway Cathelicidins Intestine
DOI10.1016/j.fsi.2020.06.059
通讯作者Huang, Wen Shu(wshuang@jmu.edu.cn) ; Chang, Ming Xian(mingxianchang@ihb.ac.cn)
英文摘要No studies systematically examined the intestinal immune response for yellow stage of European eel (Anguilla anguilla) with Aeromonas hydrophila infection by time-resolved RNA-seq. Here, we examined transcriptional profiles of the intestines at three-time points following infection with A. hydrophila. Intraperitoneal injections caused mortalities within 48 h post-injection (hpi), with the survival rate 87.5% at 24 hpi and 83.9% at 48 hpi. The result from KEGG pathway enrichment analysis showed that the immune related "cytosolic DNA-sensing pathway" was significantly enriched at the first and second time points (6 hpi and 18 hpi), with the upregulated expression of irf3, ill b, tnfaip3, cxcl8a, ap1-2, c-fos, polr3d, polr3g and polr3k both at 6 hpi and 18 hpi, but not at the third time point (36 hpi). According to the KEGG annotation, 326 immune and inflammation-related DEGs were found. The co-expression network of those 326 DEGs revealed the existence of three modules, and tlrl was found to be in the center of the biggest module which contained massive DEGs from "signal transduction" and "transport and catabolism". The c3 isoforms showed different expression pattern among the three time points, indicating a unique activation of complement systems at 18 hpi. Furthermore, two cathelicidins (aaCATH_1 and aaCATH_2) were highly up-regulated at the first two time points, and the bacterial growth inhibition assay revealed their antibacterial properties against A. hydrophila. Our data indicated the important roles of cytosolic DNA-sensing pathway, as well as transcripts including tlr1, c3, polr and cathelicidins in the intestine of A. anguilla in response to A. hydrophila infection. The present study will provide leads for functional studies of host-pathogen interactions.
WOS关键词TOLL-LIKE RECEPTORS ; TROUT ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; TRANSCRIPTOME ANALYSIS ; NUCLEAR IMPORT ; REVEALS ; SPLEEN ; COMMON ; SIGNATURES ; BACTERIA
资助项目Natural Science Foundation of Fujian Province[2018J01452] ; National Natural Science Foundation of China[31672687] ; National Natural Science Foundation of China[31873046]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号WOS:000571806800004
资助机构Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.ihb.ac.cn/handle/342005/38875]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Huang, Wen Shu; Chang, Ming Xian
作者单位1.Jimei Univ, Engn Res Ctr Modern Technol Eel Ind, Minist Educ PR China, Xiamen 361021, Peoples R China
2.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Xiong, Fan,Xiong, Jing,Wu, Ya Fang,et al. Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila[J]. FISH & SHELLFISH IMMUNOLOGY,2020,105:297-309.
APA Xiong, Fan,Xiong, Jing,Wu, Ya Fang,Cao, Lu,Huang, Wen Shu,&Chang, Ming Xian.(2020).Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila.FISH & SHELLFISH IMMUNOLOGY,105,297-309.
MLA Xiong, Fan,et al."Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila".FISH & SHELLFISH IMMUNOLOGY 105(2020):297-309.

入库方式： OAI收割

来源：水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。