Genome-Wide DNA Methylation Analysis in Male Methamphetamine Users With Different Addiction Qualities
文献类型：期刊论文
| 作者 | Liu, Liang2,3,5; Luo, Tao3,4,5; Dong, Huixi3,5; Zhang, Chenxi3,5; Liu, Tieqiao3,5; Zhang, Xiangyang1 ; Hao, Wei3,5
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| 刊名 | FRONTIERS IN PSYCHIATRY
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| 出版日期 | 2020-10-23 |
| 卷号 | 11页码:17 |
| 关键词 | methamphetamine addiction quality genome-wide DNA methylation analysis circadian entrainment pathway caveolin-2 |
| ISSN号 | 1664-0640 |
| DOI | 10.3389/fpsyt.2020.588229 |
| 通讯作者 | Zhang, Xiangyang(zhangxy@psych.ac.cn) ; Hao, Wei(weihao57@gmail.com) |
| 英文摘要 | This paper aimed to explore the genome-wide DNA methylation status of methamphetamine (MA) abusers with different qualities to addiction and to identify differentially methylated candidate genes. A total of 207 male MA abusers with an MA abuse frequency of >= 10 times and an MA abuse duration of >= 1 year were assigned to the high MA addiction quality group (HMAQ group; 168 subjects who met the diagnostic criteria for MA dependence according to the DSM-IV) or to the low MA addictive quality group (LMAQ group; 39 subjects who did not meet the criteria for MA dependence). In addition 105 healthy controls were recruited. Eight HMAQ subjects, eight LMAQ subjects, and eight healthy controls underwent genome-wide DNA methylation scans with an Infinium Human Methylation 450 array (Illumina). The differentially methylated region (DMR) data were entered into pathway analysis, and the differentially methylated position (DMP) data were screened for candidate genes and verified by MethyLight qPCR with all samples. Seven specific pathways with an abnormal methylation status were identified, including the circadian entrainment, cholinergic synapse, glutamatergic synapse, retrograde endocannabinoid signaling, GABAergic synapse, morphine addiction and PI3K-Akt signaling pathways. SLC1A6, BHLHB9, LYNX1, CAV2, and PCSK9 showed differences in their methylation levels in the three groups. Only the number of methylated copies of CAV2 was significantly higher in the LMAQ group than in the HMAQ group. Our findings suggest that the circadian entrainment pathway and the caveolin-2 gene may play key roles in MA addiction quality. Further studies on their functions and mechanisms will help us to better understand the pathogenesis of MA addiction and to explore new targets for drug intervention. |
| WOS关键词 | DORSAL STRIATUM ; DRUG-ADDICTION ; COCAINE ; EXPRESSION ; RECEPTORS ; NEURONS ; LYNX1 ; ASSOCIATION ; DISRUPTION ; METABOLISM |
| 资助项目 | National Key R&D Program of China[2017YFC1310401] ; National 973 Program[2015CB553500] |
| WOS研究方向 | Psychiatry |
| 语种 | 英语 |
| WOS记录号 | WOS:000587014300001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.psych.ac.cn/handle/311026/33473]  |
| 专题 | 心理研究所_中国科学院心理健康重点实验室 |
| 通讯作者 | Zhang, Xiangyang; Hao, Wei |
| 作者单位 | 1.Chinese Acad Sci, Inst Psychol, Beijing, Peoples R China 2.Nanjing Med Univ, Wuxi Mental Hlth Ctr, Dept Geriatr Psychiat, Wuxi, Jiangsu, Peoples R China 3.Cent South Univ, Natl Technol Inst Mental Disorders, Hunan Key Lab Psychiat & Mental Hlth, Dept Psychiat, Changsha, Peoples R China 4.Nanchang Univ, Jiangxi Mental Hosp, Dept Clin Psychiat, Nanchang, Jiangxi, Peoples R China 5.Cent South Univ, Natl Technol Inst Mental Disorders, Natl Clin Res Ctr Mental Disorders, Mental Hlth Inst,Xiangya Hosp 2, Changsha, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Liang,Luo, Tao,Dong, Huixi,et al. Genome-Wide DNA Methylation Analysis in Male Methamphetamine Users With Different Addiction Qualities[J]. FRONTIERS IN PSYCHIATRY,2020,11:17. |
| APA | Liu, Liang.,Luo, Tao.,Dong, Huixi.,Zhang, Chenxi.,Liu, Tieqiao.,...&Hao, Wei.(2020).Genome-Wide DNA Methylation Analysis in Male Methamphetamine Users With Different Addiction Qualities.FRONTIERS IN PSYCHIATRY,11,17. |
| MLA | Liu, Liang,et al."Genome-Wide DNA Methylation Analysis in Male Methamphetamine Users With Different Addiction Qualities".FRONTIERS IN PSYCHIATRY 11(2020):17. |
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来源：心理研究所
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