Chemical synthesis of Ub-AMC via ligation of peptide hydrazides
文献类型：期刊论文
| 作者 | Liang Jun2; Fang GeMin2; Huang XiuLiang1; Mei ZiQing1; Li Juan1; Tian ChangLin2; Liu Lei1
|
| 刊名 | SCIENCE CHINA-CHEMISTRY
 |
| 出版日期 | 2013 |
| 卷号 | 56 |
| 关键词 | UBIQUITIN CHAINS PROTEINS DEUBIQUITINASES ACID ligation of peptide hydrazides peptide segment condensation deubiquitinating enzymes ubiquitin |
| ISSN号 | 1674-7291 |
| 其他题名 | Chemical synthesis of Ub-AMC via ligation of peptide hydrazides |
| 英文摘要 | The C-terminal conjugate of ubiquitin with 7-amino-4-methylcoumarin (Ub-AMC) is an important probe for fluorescence-based analysis of deubiquitinating enzyme (DUB) activity. It is important to develop more efficient methods for the preparation of Ub-AMC because the currently available technology is still expensive for scaled-up production. In the present work we report an efficient strategy for total chemical synthesis of Ub-AMC through ligation of peptide hydrazides. Three peptide segments are assembled via N-to-C sequential ligation and the resulting product is converted to Ub-AMC via TCEP-mediated desulfurization. The synthetic Ub-AMC is shown to have expected biological functions through the measurement of its DUB activity. |
| 资助项目 | [National Basic Research Program of China (973 program)] ; [National Natural Science Foundation of China (NSFC)] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4935610 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/45667]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 作者单位 | 1.清华大学 2.中国科学院合肥物质科学研究院 3.University Sci & Technol China, Hefei 230026, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang Jun,Fang GeMin,Huang XiuLiang,et al. Chemical synthesis of Ub-AMC via ligation of peptide hydrazides[J]. SCIENCE CHINA-CHEMISTRY,2013,56. |
| APA | Liang Jun.,Fang GeMin.,Huang XiuLiang.,Mei ZiQing.,Li Juan.,...&Liu Lei.(2013).Chemical synthesis of Ub-AMC via ligation of peptide hydrazides.SCIENCE CHINA-CHEMISTRY,56. |
| MLA | Liang Jun,et al."Chemical synthesis of Ub-AMC via ligation of peptide hydrazides".SCIENCE CHINA-CHEMISTRY 56(2013). |
入库方式： OAI收割
来源：合肥物质科学研究院
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