Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-kappa B Signaling Pathway
文献类型：期刊论文
| 作者 | Zhou, Qinfei; Sun, Yan |
| 刊名 | CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
 |
| 出版日期 | 2020-08-19 |
| 关键词 | ABHD5 ATGL circ_cMras LAC NF-κ B signaling pathway p53 |
| ISSN号 | 1084-9785 |
| DOI | 10.1089/cbr.2020.3709 |
| 通讯作者 | Sun, Yan(nafzbr@163.com) |
| 英文摘要 | Background: Lung adenocarcinoma (LAC) is a common malignancy worldwide. Emerging findings indicated that circular RNAs possess complex capacities of gene modulation in tumorigenesis and metastasis. Nevertheless, the role of circular RNA in LAC is still largely unknown. Methods: The level of circular RNA cMras (circ_cMras), alpha-beta hydrolase domain 5 (ABHD5), and adipose triglyceride lipase (ATGL) was determined by quantitative real-time polymerase chain reaction assay. Protein levels of ABHD5, ATGL, p53, p65, and phospho-p65 (p-p65) were examined by western blot. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to detect cell proliferation in vitro. Cell apoptosis was estimated using flow cytometry. Transwell assay was used to measure cell migration and invasion in A549 and HCC827 cells. Finally, the role of circ_cMras was explored using xenograft tumor model. Results: Low levels of circ_cMras, ABHD5, and ATGL were observed in LAC tissues and cells. Upregulation of circ_cMras could hamper tumor aggression in vitro and in vivo, exhibiting as the inhibition of cell proliferation, migration, invasion, and promotion of cell apoptosis, as well as the inhibition on tumor growth in vivo. Moreover, ABHD5 deletion could overturn the effects of circ_cMras overexpression on cell behaviors in LAC cells. Furthermore, the inhibiting effects of ABHD5 on cell aggression were reversed by ATGL deficiency in vitro. Mechanically, circ_cMras/ABHD5/ATGL axis exerted its role through NF-kappa B signaling pathway in LAC cells. Conclusion: Circ_cMras exerted its function through ABHD5/ATGL axis using NF-kappa B signaling pathway in LAC, which might provide a novel insight for the diagnosis and prognosis of LAC. |
| WOS关键词 | CANCER STATISTICS ; EXPRESSION ; LIPOLYSIS |
| WOS研究方向 | Oncology ; Research & Experimental Medicine ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000586228300004 |
| 出版者 | MARY ANN LIEBERT, INC |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/105256]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Sun, Yan |
| 作者单位 | Univ Chinese Acad Sci, Dept Comprehens Med Oncol, Canc Hosp, 1 Banshan East Rd, Hangzhou 310011, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Qinfei,Sun, Yan. Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-kappa B Signaling Pathway[J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,2020. |
| APA | Zhou, Qinfei,&Sun, Yan.(2020).Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-kappa B Signaling Pathway.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS. |
| MLA | Zhou, Qinfei,et al."Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-kappa B Signaling Pathway".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS (2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。