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Effect of Sialic Acid on Mammalian Cell Culture and Protein Expression: A Potential Productivity Enhancer for Biopharmaceutical Cell Culture Processes

文献类型：期刊论文

作者Chen, Xiangsong1,2; Xiao, Shang1; Wu, Jinyong1; Yao, Jianming1,2
刊名PROCESSES
出版日期2020-11-01
卷号8
关键词HEK293 CHO sialic acid ROS lactate
DOI10.3390/pr8111449
通讯作者Yao, Jianming(jmyao@ipp.ac.cn)
英文摘要Improved productivity of the two most commonly used cell lines in the biopharmaceutical industry, such as human embryonic kidney 293 (HEK293) and Chinese hamster ovary (CHO), could reduce production costs and increase manufacturing capacity. One method for increasing protein productivity is the addition of antioxidants during the cell culture process. In this study, we examined the effect of sialic acid (SA) on one HEK293 cell line and two CHO cell lines. The addition of SA to HEK293 cell led to a higher viable cell density (VCD), viability (Via), and a lower lactate content in the later stage of cultures. Further results showed that SA reduced the reactive oxygen species (ROS), improved cell viability, reduced lactate production, and increased antibody expression by more than 20% in the later stage of the two CHO cell lines cultures. Besides, an optimized dose of SA had no significant effect on acidic variants level aggregation level, N-linked glycosylation pattern, and SA content on antibodies. These results suggest that the addition of SA can improve the productivity of biopharmaceutical cell culture processes.
WOS关键词CHO-CELLS ; LACTATE METABOLISM ; MEDIA ; ACCUMULATION ; ANTIOXIDANTS ; CONSUMPTION ; ACTIVATOR ; HISTORY ; LINES
资助项目Science and Technology Service Network Initiative of Chinese Academy of Sciences[KFJ-STS-QYZD-122]
WOS研究方向Engineering
语种英语
WOS记录号WOS:000593724000001
出版者MDPI
资助机构Science and Technology Service Network Initiative of Chinese Academy of Sciences
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/105478]  
专题中国科学院合肥物质科学研究院
通讯作者Yao, Jianming
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Plasma Phys, Hefei 230031, Peoples R China
2.Univ Sci & Technol China, Hefei 230026, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Xiangsong,Xiao, Shang,Wu, Jinyong,et al. Effect of Sialic Acid on Mammalian Cell Culture and Protein Expression: A Potential Productivity Enhancer for Biopharmaceutical Cell Culture Processes[J]. PROCESSES,2020,8.
APA Chen, Xiangsong,Xiao, Shang,Wu, Jinyong,&Yao, Jianming.(2020).Effect of Sialic Acid on Mammalian Cell Culture and Protein Expression: A Potential Productivity Enhancer for Biopharmaceutical Cell Culture Processes.PROCESSES,8.
MLA Chen, Xiangsong,et al."Effect of Sialic Acid on Mammalian Cell Culture and Protein Expression: A Potential Productivity Enhancer for Biopharmaceutical Cell Culture Processes".PROCESSES 8(2020).

入库方式： OAI收割

来源：合肥物质科学研究院

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