lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/beta-catenin signaling
文献类型：期刊论文
| 作者 | Pan, Jinchang; Fang, Shuai; Tian, Haihua; Zhou, Chengwei; Zhao, Xiaodong; Tian, Hui; He, Jinxian; Shen, Weiyu; Meng, Xiaodan; Jin, Xiaofeng |
| 刊名 | MOLECULAR CANCER
 |
| 出版日期 | 2020 |
| 卷号 | 19期号:1 |
| 关键词 | LONG NONCODING RNA HEPATOCELLULAR-CARCINOMA JPX TWIST1 CELLS PROLIFERATION EXPRESSION APOPTOSIS BIOMARKER INVASION |
| DOI | 10.1186/s12943-020-1133-9 |
| 英文摘要 | Background MicroRNAs (miRNAs) and Twist1-induced epithelial-mesenchymal transition (EMT) in cancer cell dissemination are well established, but the involvement of long noncoding RNAs (lncRNAs) in Twist1-mediated signaling remains largely unknown. Methods RT-qPCR and western blotting were conducted to detect the expression levels of lncRNA JPX and Twist1 in lung cancer cell lines and tissues. The impact of JPX on Twist1 expression, cell growth, invasion, apoptosis, and in vivo tumor growth were investigated in lung cancer cells by western blotting, rescue experiments, colony formation assay, flow cytometry, and xenograft animal experiment. Results We observed that lncRNA JPX was upregulated in lung cancer metastatic tissues and was closely correlated with tumor size and an advanced stage. Functionally, JPX promoted lung cancer cell proliferation in vitro and facilitated lung tumor growth in vivo. Additionally, JPX upregulated Twist1 by competitively sponging miR-33a-5p and subsequently induced EMT and lung cancer cell invasion. Interestingly, JPX and Twist1 were coordinately upregulated in lung cancer tissues and cells. Mechanically, the JPX/miR-33a-5p/Twist1 axis participated in EMT progression by activating Wnt/beta-catenin signaling. Conclusions These findings suggest that lncRNA JPX, a mediator of Twist1 signaling, could predispose lung cancer cells to metastasis and may serve as a potential target for targeted therapy. |
| 学科主题 | Biochemistry & Molecular Biology ; Oncology |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/20171]  |
| 专题 | 2020专题 2020专题_期刊论文 |
| 作者单位 | 1.Gong, ZH (corresponding author), Ningbo Univ, Sch Med, Dept Biochem & Mol Biol, Ningbo 315211, Peoples R China. 2.Gong, ZH (corresponding author), Ningbo Univ, Sch Med, Zhejiang Prov Key Lab Pathophysiol, Ningbo 315211, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Pan, Jinchang,Fang, Shuai,Tian, Haihua,et al. lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/beta-catenin signaling[J]. MOLECULAR CANCER,2020,19(1). |
| APA | Pan, Jinchang.,Fang, Shuai.,Tian, Haihua.,Zhou, Chengwei.,Zhao, Xiaodong.,...&Gong, Zhaohui.(2020).lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/beta-catenin signaling.MOLECULAR CANCER,19(1). |
| MLA | Pan, Jinchang,et al."lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/beta-catenin signaling".MOLECULAR CANCER 19.1(2020). |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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