Textured nanofibrils drive microglial phenotype
文献类型：期刊论文
| 作者 | Song, Qin; Pifferi, Simone; Shi, Lin; Chen, Chuntao; Zaccaria, Remo Proietti; Menini, Anna; Cao, Jianping; Zhang, Qi; Torre, Vincent |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2020 |
| 卷号 | 257 |
| 关键词 | BACTERIAL CELLULOSE CHANNEL EXPRESSION K+ PHYSIOLOGY MIGRATION HEALTH CELLS |
| DOI | 10.1016/j.biomaterials.2020.120177 |
| 英文摘要 | Microglia are highly plastic cells that change their properties in response to their microenvironment. By using immunofluorescence, live-cell imaging, electrophysiological recordings and RNA sequencing, we investigated the regulation of modified bacterial cellulose (mBC) nanofibril substrates on microglial properties. We demonstrate that mBC substrates induce ramified microglia with constantly extending and retracting processes, reminiscent of what is observed in vivo. Patch-clamp recordings show that microglia acquire a more negative resting membrane potential and have increased inward rectifier K+ currents, caused by an upregulation of Kir2.1 channels. Transcriptome analysis shows upregulation of genes involved in the immune response and down-regulation of genes linked to cell adhesion and cell motion. Furthermore, Arp2/3 complex activation and integrin-mediated signaling modulate microglial morphology and motility. Our studies demonstrate that mBC nanofibril substrates modulate microglial phenotype, paving the way for a microglia-material interface that may be very valuable for anti-neuroinflammatory drug screening. |
| 学科主题 | Engineering ; Materials Science |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/20462]  |
| 专题 | 2020专题 2020专题_期刊论文 |
| 作者单位 | 1.Zhang, Q 2.Torre, V (corresponding author), Int Sch Adv Studies SISSA, I-34136 Trieste, Italy. 3.Torre, V (corresponding author), Soochow Univ, State Key Lab Radiat Med & Protect, Med Coll, Sch Radiat Med & Protect, Suzhou, Jiangsu, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Song, Qin,Pifferi, Simone,Shi, Lin,et al. Textured nanofibrils drive microglial phenotype[J]. BIOMATERIALS,2020,257. |
| APA | Song, Qin.,Pifferi, Simone.,Shi, Lin.,Chen, Chuntao.,Zaccaria, Remo Proietti.,...&Torre, Vincent.(2020).Textured nanofibrils drive microglial phenotype.BIOMATERIALS,257. |
| MLA | Song, Qin,et al."Textured nanofibrils drive microglial phenotype".BIOMATERIALS 257(2020). |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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