Improving the performance of upconversion nanoprobe-based lateral flow immunoassays by supramolecular self-assembly core/shell strategies
文献类型：期刊论文
| 作者 | Huang, Zhen; Liu, Yihui; Chen, Yuan; Xiong, Qirong; Wang, Yuhui; Duan, Hongwei; Lai, Weihua |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2020 |
| 卷号 | 318 |
| 关键词 | PHOSPHOR REPORTERS IN-VITRO NANOPARTICLES LUMINESCENCE SURFACE NANOCRYSTALS PLATFORM ASSAY PAIR YB3+ |
| DOI | 10.1016/j.snb.2020.128233 |
| 英文摘要 | Upconversion nanoparticles (UCNPs) have been used as probes in lateral flow immunoassays (LFIAs), because the probe provides an excellent signal-to-noise ratio by eliminating background-fluorescence from biological samples. However, the low photoluminescence efficiency, unfavorable hydrophilic modification and poor antibody conjugation are insurmountable challenges for the application of UCNPs to LFIAs. In this work, we have made a breakthrough by integrating friendly ligand-exchange and supramolecular self-assembly strategies. It is demonstrated that the Cucurbit[7]uril-based hydrophilic modification strategy can improve the photoluminescence of UCNPs by two-fold and significantly improves the stability of the UCNPs. More importantly, the supramolecular self-assembly strategy, which is 10 times more efficient than the carboxyl-amino reaction for antibody conjugation. The UCNPs were successfully applied to sandwich and competitive LFIAs for the sensitive detection of a pathogenic bacteria (Escherichia coli O157:H7) and micro-molecule (danofloxacin). In comparison with existing methods, we demonstrate that this novel method could enhance the sensitivity by 40 times, expand the detection range by one order of magnitude, and reduce the costs by 20 %-40%. This new method not only inherits the excellent matrix tolerance of UCNPs for the detection of bio-samples but also improves the performance of LFIAs with regards to their sensitivity, quantitative range, and cost. |
| 学科主题 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/20554]  |
| 专题 | 2020专题 2020专题_期刊论文 |
| 作者单位 | 1.Duan, HW (corresponding author), Nanyang Technol Univ, Sch Chem & Biomed Engn, 70 Nanyang Dr,N1-3,B3-14, Singapore 637457, Singapore. 2.Wang, YH (corresponding author), Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Key Lab Graphene Technol & Applicat Zhejiang Prov, 1219 Zhongguan West Rd, Ningbo 315201, Peoples R China. 3.Lai, WH (corresponding author), Nanchang Univ, State Key Lab Food Sci & Technol, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Huang, Zhen,Liu, Yihui,Chen, Yuan,et al. Improving the performance of upconversion nanoprobe-based lateral flow immunoassays by supramolecular self-assembly core/shell strategies[J]. SENSORS AND ACTUATORS B-CHEMICAL,2020,318. |
| APA | Huang, Zhen.,Liu, Yihui.,Chen, Yuan.,Xiong, Qirong.,Wang, Yuhui.,...&Lai, Weihua.(2020).Improving the performance of upconversion nanoprobe-based lateral flow immunoassays by supramolecular self-assembly core/shell strategies.SENSORS AND ACTUATORS B-CHEMICAL,318. |
| MLA | Huang, Zhen,et al."Improving the performance of upconversion nanoprobe-based lateral flow immunoassays by supramolecular self-assembly core/shell strategies".SENSORS AND ACTUATORS B-CHEMICAL 318(2020). |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。