Rac1 Promotes Cell Motility by Controlling Cell Mechanics in Human Glioblastoma
文献类型：期刊论文
| 作者 | Xu, Jing; Galvanetto, Nicola; Nie, Jihua; Yang, Yili; Torre, Vincent |
| 刊名 | CANCERS
 |
| 出版日期 | 2020 |
| 卷号 | 12期号:6 |
| 关键词 | ADJUVANT TEMOZOLOMIDE ACTIN-FILAMENTS ARP2/3 COMPLEX RHO GTPASES CANCER MICROTUBULES RADIOTHERAPY CONCOMITANT RESISTANT ROLES |
| DOI | 10.3390/cancers12061667 |
| 英文摘要 | The failure of existing therapies in treating human glioblastoma (GBM) mostly is due to the ability of GBM to infiltrate into healthy regions of the brain; however, the relationship between cell motility and cell mechanics is not well understood. Here, we used atomic force microscopy (AFM), live-cell imaging, and biochemical tools to study the connection between motility and mechanics in human GBM cells. It was found thatRac1 inactivation by genomic silencing and inhibition with EHT 1864 reduced cell motility, inhibited cell ruffles, and disrupted the dynamics of cytoskeleton organization and cell adhesion. These changes were correlated with abnormal localization of myosin IIa and a rapid suppression of the phosphorylation of Erk1/2. At the same time, AFM measurements of the GBM cells revealed a significant increase in cell elasticity and viscosity following Rac1 inhibition. These results indicate that mechanical properties profoundly affect cell motility and may play an important role in the infiltration of GBM. It is conceivable that the mechanical characters might be used as markers for further surgical and therapeutical interventions. |
| 学科主题 | Oncology |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/20756]  |
| 专题 | 2020专题 2020专题_期刊论文 |
| 作者单位 | 1.Torre, V (corresponding author), Int Sch Adv Studies SISSA, I-34136 Trieste, Italy. 2.Yang, YL 3.Torre, V (corresponding author), Int Sch Adv Studies SISSA, Joint Lab Biophys & Translat Med, Suzhou Inst Syst Med ISM, Suzhou 215123, Peoples R China. 4.Torre, V (corresponding author), Soochow Univ, Sch Radiat Med & Protect, State Key Lab Radiat Med & Protect, Med Coll, Suzhou 215123, Peoples R China. 5.Torre, V (corresponding author), Chinese Acad Sci, Cixi Inst Biomed Engn, Ningbo Inst Mat Technol & Engn, Ningbo 315201, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Xu, Jing,Galvanetto, Nicola,Nie, Jihua,et al. Rac1 Promotes Cell Motility by Controlling Cell Mechanics in Human Glioblastoma[J]. CANCERS,2020,12(6). |
| APA | Xu, Jing,Galvanetto, Nicola,Nie, Jihua,Yang, Yili,&Torre, Vincent.(2020).Rac1 Promotes Cell Motility by Controlling Cell Mechanics in Human Glioblastoma.CANCERS,12(6). |
| MLA | Xu, Jing,et al."Rac1 Promotes Cell Motility by Controlling Cell Mechanics in Human Glioblastoma".CANCERS 12.6(2020). |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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