Dually enhanced homogenous synthesis of molybdophosphate by hybridization chain reaction and enzyme nanotags for the electrochemical bioassay of carcinoembryonic antigen
文献类型：期刊论文
| 作者 | Cai, Xiaolei; Lv, Fuhui; Lai, Guosong; Fu, Li; Lin, Cheng-Te; Yu, Aimin |
| 刊名 | MICROCHIMICA ACTA
 |
| 出版日期 | 2020 |
| 卷号 | 187期号:6 |
| 关键词 | GOLD NANOPARTICLES CANCER BIOMARKER AMPLIFICATION DNA IMMUNOASSAY APTASENSOR STRATEGY |
| DOI | 10.1007/s00604-020-04342-3 |
| 英文摘要 | A magnetic bead (MB)-based sandwich biorecognition reactions is combined with a gold nanoprobe-induced homogenous synthesis of molybdophosphate to develop a novel bioassay method for the electrochemical detection of the tumor biomarker of carcinoembryonic antigen (CEA). The nanoprobe is prepared through the specific loading of numerous alkaline phosphatase (ALP)-functionalized gold nanoparticles (Au NPs) on a double-stranded DNA (dsDNA) produced by the CEA aptamer-triggered hybridization chain reaction (HCR). Both the large amounts of PO43- produced by the ALP catalytic hydrolysis of pyrophosphate and the phosphate backbones of dsDNA can react with the added MoO42- to generate electroactive molybdophosphates. So, the gold nanoprobe was used for signal tracing of the sandwich bioassay of CEA at a constructed antibody-functionalized MB platform. The sensitive electrochemical measurement of molybdophosphate produced from the quantitatively captured nanoprobes at a carbon nanotube-modified electrode (measured at about 0.12 V vs. Ag/AgCl, 3 M KCl) enabled the convenient signal transduction of the method. Due to the dually enhanced synthesis of molybdophosphate by the HCR and multi-enzyme Au NP nanotags, this method shows a wide linear range from 0.05 pg mL(-1) to 10 ng mL(-1) along with a low detection limit of 0.027 pg mL(-1). In addition, the MB-based biorecognition reaction and the homogeneous synthesis of molybdophosphate are much convenient in manipulations. These excellent performances decide the extensive application potentials of the method. |
| 学科主题 | Chemistry |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/20780]  |
| 专题 | 2020专题 2020专题_期刊论文 |
| 作者单位 | 1.Lai, GS 2.Yu, AM (corresponding author), Hubei Normal Univ, Dept Chem, Hubei Key Lab Pollutant Anal & Reuse Technol, Huangshi 435002, Hubei, Peoples R China. 3.Yu, AM (corresponding author), Swinburne Univ Technol, Fac Sci Engn & Technol, Fac Chem & Biotechnol, Hawthorn, Vic 3122, Australia. |
| 推荐引用方式 GB/T 7714 | Cai, Xiaolei,Lv, Fuhui,Lai, Guosong,et al. Dually enhanced homogenous synthesis of molybdophosphate by hybridization chain reaction and enzyme nanotags for the electrochemical bioassay of carcinoembryonic antigen[J]. MICROCHIMICA ACTA,2020,187(6). |
| APA | Cai, Xiaolei,Lv, Fuhui,Lai, Guosong,Fu, Li,Lin, Cheng-Te,&Yu, Aimin.(2020).Dually enhanced homogenous synthesis of molybdophosphate by hybridization chain reaction and enzyme nanotags for the electrochemical bioassay of carcinoembryonic antigen.MICROCHIMICA ACTA,187(6). |
| MLA | Cai, Xiaolei,et al."Dually enhanced homogenous synthesis of molybdophosphate by hybridization chain reaction and enzyme nanotags for the electrochemical bioassay of carcinoembryonic antigen".MICROCHIMICA ACTA 187.6(2020). |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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