A PNA-DNA(2) Triple-Helix Molecular Switch-Based Colorimetric Sensor for Sensitive and Specific Detection of microRNAs from Cancer Cells
文献类型：期刊论文
| 作者 | Xu, Mengjia; Fu, Pan; Xing, Shu; Zhao, Yang; Zhao, Chao |
| 刊名 | CHEMBIOCHEM
 |
| 出版日期 | 2020 |
| 卷号 | 21期号:18页码:2667-2675 |
| 关键词 | ULTRASENSITIVE DETECTION DNA PNA AMPLIFICATION HYBRIDIZATION FLUORESCENT RECOGNITION UNIVERSAL GRAPHENE INVASION |
| DOI | 10.1002/cbic.202000155 |
| 英文摘要 | Peptide nucleic acids (PNAs), the synthetic DNA mimics that can bind to oligonucleotides to form duplexes, triplexes, and quadruplexes, could be advantageous as probes for nucleic acid sequences owing to their unique physicochemical and biochemical properties. We have found that a homopurine PNA strand could bind to two homopyrimidine DNA strands to form a PNA-DNA(2) triplex. Moreover, the cyanine dye DiSC(2)(5) could bind with high affinity to this triplex and cause a noticeable color change. On the basis of this phenomenon, we have designed a label-free colorimetric sensing platform for miRNAs from cancer cells by using a PNA-DNA(2) triple-helix molecular switch (THMS) and DiSC(2)(5). This sensing platform can detect miRNA-21 specifically with a detection limit of 0.18 nM, which is comparable to that of the THMS-mediated fluorescence sensing platform. Moreover, this colorimetric platform does not involve any chemical modification or enzymatic signal amplification, which boosts its applicability and availability at the point of care in resource-limited settings. The universality of this approach can be simply achieved by altering the sequences of the probe DNA for specific targets. |
| 学科主题 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/20782]  |
| 专题 | 2020专题 2020专题_期刊论文 |
| 作者单位 | 1.Zhao, C (corresponding author), Chinese Acad Sci, Cixi Inst Biomed Engn, Ningbo Inst Mat Technol & Engn, Ningbo 315201, Peoples R China. 2.Zhao, Y (corresponding author), Ningbo Univ, Coll Sci & Technol, Ningbo 315212, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Xu, Mengjia,Fu, Pan,Xing, Shu,et al. A PNA-DNA(2) Triple-Helix Molecular Switch-Based Colorimetric Sensor for Sensitive and Specific Detection of microRNAs from Cancer Cells[J]. CHEMBIOCHEM,2020,21(18):2667-2675. |
| APA | Xu, Mengjia,Fu, Pan,Xing, Shu,Zhao, Yang,&Zhao, Chao.(2020).A PNA-DNA(2) Triple-Helix Molecular Switch-Based Colorimetric Sensor for Sensitive and Specific Detection of microRNAs from Cancer Cells.CHEMBIOCHEM,21(18),2667-2675. |
| MLA | Xu, Mengjia,et al."A PNA-DNA(2) Triple-Helix Molecular Switch-Based Colorimetric Sensor for Sensitive and Specific Detection of microRNAs from Cancer Cells".CHEMBIOCHEM 21.18(2020):2667-2675. |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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