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  5. 期刊论文
Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production

文献类型：期刊论文

作者Li, Tianpei7,8,9,10; Jiang, Qiuyao10; Huang, Jiafeng6,10; Aitchison, Catherine M.4,5; Huang, Fang10; Yang, Mengru; Dykes, Gregory F.10; He, Hai-Lun6; Wang, Qiang3,7; Sprick, Reiner Sebastian4,5
刊名NATURE COMMUNICATIONS
出版日期2020-10-28
卷号11期号:1页码:10
ISSN号2041-1723
DOI10.1038/s41467-020-19280-0
通讯作者Wang, Qiang(wangqiang@henu.edu.cn) ; Liu, Lu-Ning(luning.liu@liverpool.ac.uk)
英文摘要Compartmentalization is a ubiquitous building principle in cells, which permits segregation of biological elements and reactions. The carboxysome is a specialized bacterial organelle that encapsulates enzymes into a virus-like protein shell and plays essential roles in photosynthetic carbon fixation. The naturally designed architecture, semi-permeability, and catalytic improvement of carboxysomes have inspired rational design and engineering of new nanomaterials to incorporate desired enzymes into the protein shell for enhanced catalytic performance. Here, we build large, intact carboxysome shells (over 90nm in diameter) in the industrial microorganism Escherichia coli by expressing a set of carboxysome protein-encoding genes. We develop strategies for enzyme activation, shell self-assembly, and cargo encapsulation to construct a robust nanoreactor that incorporates catalytically active [FeFe]-hydrogenases and functional partners within the empty shell for the production of hydrogen. We show that shell encapsulation and the internal microenvironment of the new catalyst facilitate hydrogen production of the encapsulated oxygen-sensitive hydrogenases. The study provides insights into the assembly and formation of carboxysomes and paves the way for engineering carboxysome shell-based nanoreactors to recruit specific enzymes for diverse catalytic reactions. The extreme oxygen sensitive character of hydrogenases is a longstanding issue for hydrogen production in bacteria. Here, the authors build carboxysome shells in E. coli and incorporate catalytically active hydrogenases and functional partners within the empty shell for the production of hydrogen.
WOS关键词HALOTHIOBACILLUS-NEAPOLITANUS CARBOXYSOMES ; CARBONIC-ANHYDRASE ; SHELL PROTEIN ; MICROCOMPARTMENTS ; ORGANIZATION ; PROCHLOROCOCCUS ; IDENTIFICATION ; EVOLUTION ; FIXATION ; REVEALS
资助项目Royal Society University Research Fellowship[UF120411] ; Royal Society University Research Fellowship[URF\R\180030] ; Royal Society Fellow Enhancement Awards[RGF\EA\181061] ; Royal Society Fellow Enhancement Awards[RGF\EA\180233] ; Biotechnology and Biological Sciences Research Council[BB/M024202/1] ; Biotechnology and Biological Sciences Research Council[BB/R003890/1] ; British Council Newton Fund Grant[201703780114] ; British Council Newton Fund Grant[201806370307] ; Leverhulme Trust[ECF-2016-778] ; National Natural Science Foundation of China[91851103] ; National Natural Science Foundation of China[31770128] ; 111 Project[D16014] ; UK Engineering and Physical Sciences Research Council (EPSRC)[EP/N004884/1]
WOS研究方向Science & Technology - Other Topics
语种英语
出版者NATURE RESEARCH
WOS记录号WOS:000588060400005
资助机构Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC)
源URL[http://ir.ihb.ac.cn/handle/342005/39259]  
专题水生生物研究所_藻类生物学及应用研究中心_期刊论文
通讯作者Wang, Qiang; Liu, Lu-Ning
作者单位1.Ocean Univ China, Frontiers Sci Ctr Deep Ocean Multispheres & Earth, Qingdao 266003, Peoples R China
2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China
4.Univ Liverpool, Dept Chem, Liverpool L7 3NY, Merseyside, England
5.Univ Liverpool, Mat Innovat Factory, Liverpool L7 3NY, Merseyside, England
6.Cent South Univ, Sch Life Sci, Changsha, Peoples R China
7.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Peoples R China
8.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
9.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
10.Univ Liverpool, Inst Syst Mol & Integrat Biol, Liverpool L69 7ZB, Merseyside, England
推荐引用方式
GB/T 7714		 Li, Tianpei,Jiang, Qiuyao,Huang, Jiafeng,et al. Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production[J]. NATURE COMMUNICATIONS,2020,11(1):10.
APA Li, Tianpei.,Jiang, Qiuyao.,Huang, Jiafeng.,Aitchison, Catherine M..,Huang, Fang.,...&Liu, Lu-Ning.(2020).Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production.NATURE COMMUNICATIONS,11(1),10.
MLA Li, Tianpei,et al."Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production".NATURE COMMUNICATIONS 11.1(2020):10.

入库方式： OAI收割

来源：水生生物研究所

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