Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae
文献类型：期刊论文
| 作者 | Zhang, Yu2; Pan, Yufang1; Ding, Wei2; Hu, Hanhua1; Liu, Jin2 |
| 刊名 | GLOBAL CHANGE BIOLOGY BIOENERGY
 |
| 出版日期 | 2020-11-10 |
| 页码 | 16 |
| 关键词 | biofuel diacylglycerol acyltransferase genetic manipulation oleaginous alga Phaeodactylum tricornutum subcellular localization triacylglycerol |
| ISSN号 | 1757-1693 |
| DOI | 10.1111/gcbb.12771 |
| 通讯作者 | Liu, Jin(gjinliu@pku.edu.cn) |
| 英文摘要 | Oleaginous algae have the ability to synthesize a high level of triacylglycerol (TAG) and are considered as the next-generation feedstock for biofuel production. Manipulating algal lipid biosynthetic pathways has potential to overproduce TAG and represents a feasible way toward bringing down algae-derived biofuels production cost. Here we dissected functional roles and engineering potential of six diacylglycerol acyltransferase (DGAT) genes from the marine alga Phaeodactylum tricornutum. PtDGAT1, PtDGAT2B, and PtDGAT3, residing at the chloroplast endoplasmic reticulum (ER) readily for utilizing both chloroplast- and ER-derived diacylglycerol, were transcriptionally correlated with TAG accumulation. Heterologous expression in yeast, in vitro assay and overexpression in P. tricornutum all supported that PtDGAT1 surpassed the other five PtDGATs in synthesizing TAG. Compared to wild type P. tricornutum, the PtDGAT1-overexpressing strain produced more than doubled TAG and total lipids, which reached 57% and 73% of dry weight, respectively, record-high levels ever achieved in this alga. Our results demonstrated the strategy of screening proper engineering targets and manipulating a single gene to pull carbon flux to lipids for TAG hyper-accumulation without growth compromise. The engineered alga with a great trait improvement may serve as a potent lipid producer for production uses. |
| WOS关键词 | FATTY-ACID BIOSYNTHESIS ; PHAEODACTYLUM-TRICORNUTUM ; CHLAMYDOMONAS-REINHARDTII ; TRIACYLGLYCEROL ACCUMULATION ; PHOSPHORUS STARVATION ; GREEN MICROALGA ; ACYL-COENZYME ; OIL SYNTHESIS ; IDENTIFICATION ; MEMBRANE |
| 资助项目 | National Natural Science Foundation of China[31571807] ; National Natural Science Foundation of China[41976119] ; Peking University CCUS by BHP Billiton |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 语种 | 英语 |
| WOS记录号 | WOS:000587924700001 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/39289]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 通讯作者 | Liu, Jin |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China 2.Peking Univ, Coll Engn, Lab Algae Biotechnol & Innovat, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yu,Pan, Yufang,Ding, Wei,et al. Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae[J]. GLOBAL CHANGE BIOLOGY BIOENERGY,2020:16. |
| APA | Zhang, Yu,Pan, Yufang,Ding, Wei,Hu, Hanhua,&Liu, Jin.(2020).Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae.GLOBAL CHANGE BIOLOGY BIOENERGY,16. |
| MLA | Zhang, Yu,et al."Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae".GLOBAL CHANGE BIOLOGY BIOENERGY (2020):16. |
入库方式： OAI收割
来源：水生生物研究所
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