Germination-Arrest Bacillus subtilis Spores as An Oral Delivery Vehicle of Grass Carp Reovirus (GCRV) Vp7 Antigen Augment Protective Immunity in Grass Carp (Ctenopharyngodon idella)
文献类型：期刊论文
| 作者 | Sun, Rui3; Zhang, Miao3; Chen, Hui2; Wei, Yao3; Ning, Degang1,3 |
| 刊名 | GENES
 |
| 出版日期 | 2020-11-01 |
| 卷号 | 11期号:11页码:15 |
| 关键词 | Bacillus subtilis germination-arrest spore surface display GCRV oral vaccination protective immunity grass carp |
| DOI | 10.3390/genes11111351 |
| 通讯作者 | Ning, Degang(ningdegang@ihb.ac.cn) |
| 英文摘要 | Oral vaccination is a practical method for the active immunization of farmed fish in the matter of animal welfare and handling costs. However, it always shows insufficient protective immunity, mainly due to antigen degradation in the gastrointestinal tract (GIT). Bacillus subtilis spores have been shown to be able to protect surface-display heterologous antigens against degradation. Neverthless, the spores can germinate in GIT, which causes loss of the antigens with spore coat disassembly. Here, we developed a novel surface display system using the B. subtilis spore coat proteins CotB and CotC as anchors for the heterogenous antigen, and the germination-controlling genes cwlJ and sleB as the ectopic integration sites for the fusion genes. Using this display system, we engineered germination-arrest spores displaying the model antigen Vp7 of grass carp reovirus (GCRV) on their surface. Oral vaccination of the engineered spores could confer immune protection against GCRV in grass carp (Ctenopharyngodon idella) via eliciting adaptive humoral and cellular immune responses. Most importantly, the germination-arrest spores were shown to significantly augment immunogenicity and protection above the engineered spores based on the existing surface display system. Therefore, the presently reported antigen expression strategy opens new and promising avenues for developing oral vaccines for the immunization of farmed fish species. |
| WOS关键词 | TUMOR-NECROSIS-FACTOR ; PROTEIN ; SURFACE ; GENE ; REPERTOIRES ; INDUCTION ; VACCINES ; VIRUS |
| 资助项目 | Key Research and Development Project of Jiangsu[BE2015330] ; Agricultural Independent Innovation Project of Jiangsu[CX(17)2027] ; Key Research and Development Project of Huaian[HAN201811] |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000593835200001 |
| 出版者 | MDPI |
| 资助机构 | Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/39376]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 通讯作者 | Ning, Degang |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, CAS Key Lab Algal Biol, Wuhan 430072, Peoples R China 2.China Jiangsu Ctr Aquat Anim Dis Prevent & Contro, Nanjing 210036, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Huaian Res Ctr, Huaian 223005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Rui,Zhang, Miao,Chen, Hui,et al. Germination-Arrest Bacillus subtilis Spores as An Oral Delivery Vehicle of Grass Carp Reovirus (GCRV) Vp7 Antigen Augment Protective Immunity in Grass Carp (Ctenopharyngodon idella)[J]. GENES,2020,11(11):15. |
| APA | Sun, Rui,Zhang, Miao,Chen, Hui,Wei, Yao,&Ning, Degang.(2020).Germination-Arrest Bacillus subtilis Spores as An Oral Delivery Vehicle of Grass Carp Reovirus (GCRV) Vp7 Antigen Augment Protective Immunity in Grass Carp (Ctenopharyngodon idella).GENES,11(11),15. |
| MLA | Sun, Rui,et al."Germination-Arrest Bacillus subtilis Spores as An Oral Delivery Vehicle of Grass Carp Reovirus (GCRV) Vp7 Antigen Augment Protective Immunity in Grass Carp (Ctenopharyngodon idella)".GENES 11.11(2020):15. |
入库方式： OAI收割
来源：水生生物研究所
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