Characterization of Streptococcus iniae ghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)
文献类型:期刊论文
作者 | Wang, Qishuo4,5,6; Zhang, Chao3; Xu, La2; Chen, Jiakang5,6; Wang, Xuepeng1,5,6 |
刊名 | AQUACULTURE RESEARCH |
出版日期 | 2020-11-28 |
页码 | 10 |
ISSN号 | 1355-557X |
关键词 | bacterial ghosts challenge immune response Streptococcus iniae tilapia |
DOI | 10.1111/are.14990 |
通讯作者 | Wang, Xuepeng(xpwang@sdau.edu.cn) |
英文摘要 | Streptococcus iniae (S. iniae) is a Gram-positive bacterium associated with disease in several commercial species and other animals including humans. This zoonotic pathogen has led to high worldwide morbidity and mortality and huge economic losses in aquaculture. Here a S. iniae ghost (SIG) vaccine was successfully generated with a chemical method, and its immunogenicity and protective efficacy were examined in this study. The results showed that the titres of immunoglobulin M (IgM) in serum were significantly higher in group A (SIG-immunized) than group B (formalin-inactivated S. iniae, FSI-immunized), which was significantly higher than the control group (PBS-treated). Also, the phagocytic activity was significantly higher in group A than group B, which was significantly higher than the control group. Moreover, AKP activity and lysozyme activity of serum in group A were significantly higher than group B, which was significantly higher than the control group. Also, IL-1 beta, IFN-gamma and HSP70 in the head kidney of fish immunized with SIG and FSI showed significantly higher than the control group throughout the whole experimental period. In addition, IL-1 beta and IFN-gamma in the spleen of fish immunized with SIG showed significantly higher than that of in the FSI-immunized fish. Most important, the survival rate and relative per cent survival of group A were higher than group B, and both of them were all significantly more protected against the S. iniae challenge compared to the PBS-treated group. These results suggest that immunization with SIG induces immune responses and provides protection against a virulent S. iniae challenge. |
WOS关键词 | IMMUNE-RESPONSES ; EDWARDSIELLA-PISCICIDA ; BACTERIAL GHOSTS ; INFECTION ; PROTEIN ; INNATE ; RESISTANCE ; AGALACTIAE ; DEFENSE ; MUTANT |
资助项目 | Open Fund of Shandong Key Laboratory of Disease Control in Mariculture[KF201803] |
WOS研究方向 | Fisheries |
语种 | 英语 |
出版者 | WILEY |
WOS记录号 | WOS:000593032500001 |
资助机构 | Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture |
源URL | [http://ir.ihb.ac.cn/handle/342005/39388] |
专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
通讯作者 | Wang, Xuepeng |
作者单位 | 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China 2.Marine Biol Inst Shandong Prov, Shandong Key Lab Dis Control Maricuiture, Qingdao, Peoples R China 3.Chongqing Three Gorges Vocat Coll, Chongqing, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China 5.Shandong Agr Univ, Shandong Prov Engn Technol Res Ctr Anim Dis Contr, Tai An, Shandong, Peoples R China 6.Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Qishuo,Zhang, Chao,Xu, La,et al. Characterization of Streptococcus iniae ghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)[J]. AQUACULTURE RESEARCH,2020:10. |
APA | Wang, Qishuo,Zhang, Chao,Xu, La,Chen, Jiakang,&Wang, Xuepeng.(2020).Characterization of Streptococcus iniae ghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus).AQUACULTURE RESEARCH,10. |
MLA | Wang, Qishuo,et al."Characterization of Streptococcus iniae ghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)".AQUACULTURE RESEARCH (2020):10. |
入库方式: OAI收割
来源:水生生物研究所
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