中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Characterization of Streptococcus iniae ghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)

文献类型:期刊论文

作者Wang, Qishuo4,5,6; Zhang, Chao3; Xu, La2; Chen, Jiakang5,6; Wang, Xuepeng1,5,6
刊名AQUACULTURE RESEARCH
出版日期2020-11-28
页码10
ISSN号1355-557X
关键词bacterial ghosts challenge immune response Streptococcus iniae tilapia
DOI10.1111/are.14990
通讯作者Wang, Xuepeng(xpwang@sdau.edu.cn)
英文摘要Streptococcus iniae (S. iniae) is a Gram-positive bacterium associated with disease in several commercial species and other animals including humans. This zoonotic pathogen has led to high worldwide morbidity and mortality and huge economic losses in aquaculture. Here a S. iniae ghost (SIG) vaccine was successfully generated with a chemical method, and its immunogenicity and protective efficacy were examined in this study. The results showed that the titres of immunoglobulin M (IgM) in serum were significantly higher in group A (SIG-immunized) than group B (formalin-inactivated S. iniae, FSI-immunized), which was significantly higher than the control group (PBS-treated). Also, the phagocytic activity was significantly higher in group A than group B, which was significantly higher than the control group. Moreover, AKP activity and lysozyme activity of serum in group A were significantly higher than group B, which was significantly higher than the control group. Also, IL-1 beta, IFN-gamma and HSP70 in the head kidney of fish immunized with SIG and FSI showed significantly higher than the control group throughout the whole experimental period. In addition, IL-1 beta and IFN-gamma in the spleen of fish immunized with SIG showed significantly higher than that of in the FSI-immunized fish. Most important, the survival rate and relative per cent survival of group A were higher than group B, and both of them were all significantly more protected against the S. iniae challenge compared to the PBS-treated group. These results suggest that immunization with SIG induces immune responses and provides protection against a virulent S. iniae challenge.
WOS关键词IMMUNE-RESPONSES ; EDWARDSIELLA-PISCICIDA ; BACTERIAL GHOSTS ; INFECTION ; PROTEIN ; INNATE ; RESISTANCE ; AGALACTIAE ; DEFENSE ; MUTANT
资助项目Open Fund of Shandong Key Laboratory of Disease Control in Mariculture[KF201803]
WOS研究方向Fisheries
语种英语
出版者WILEY
WOS记录号WOS:000593032500001
资助机构Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture
源URL[http://ir.ihb.ac.cn/handle/342005/39388]  
专题水生生物研究所_淡水生态学研究中心_期刊论文
通讯作者Wang, Xuepeng
作者单位1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China
2.Marine Biol Inst Shandong Prov, Shandong Key Lab Dis Control Maricuiture, Qingdao, Peoples R China
3.Chongqing Three Gorges Vocat Coll, Chongqing, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
5.Shandong Agr Univ, Shandong Prov Engn Technol Res Ctr Anim Dis Contr, Tai An, Shandong, Peoples R China
6.Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Wang, Qishuo,Zhang, Chao,Xu, La,et al. Characterization of Streptococcus iniae ghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)[J]. AQUACULTURE RESEARCH,2020:10.
APA Wang, Qishuo,Zhang, Chao,Xu, La,Chen, Jiakang,&Wang, Xuepeng.(2020).Characterization of Streptococcus iniae ghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus).AQUACULTURE RESEARCH,10.
MLA Wang, Qishuo,et al."Characterization of Streptococcus iniae ghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)".AQUACULTURE RESEARCH (2020):10.

入库方式: OAI收割

来源:水生生物研究所

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