中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 水生生物研究所
  3. 中科院水生所知识产出（2009年前）
  4. 藻类生物学及应用研究中心
  5. 期刊论文
Understanding the Differences in the Growth and Toxin Production of Anatoxin-Producing Cuspidothrix issatschenkoi Cultured with Inorganic and Organic N Sources from a New Perspective: Carbon/Nitrogen Metabolic Balance

文献类型：期刊论文

作者Tao, Siyi2,3; Wang, Suqin1; Song, Lirong3; Gan, Nanqin3
刊名TOXINS
出版日期2020-11-01
卷号12期号:11页码:20
关键词anatoxin-a Cuspidothrix issatschenkoi nitrogen sources urea L-alanine C N ratio
DOI10.3390/toxins12110724
通讯作者Gan, Nanqin(gannq@ihb.ac.cn)
英文摘要Cyanotoxins are the underlying cause of the threat that globally pervasive Cyanobacteria Harmful algal blooms (CyanoHABs) pose to humans. Major attention has been focused on the cyanobacterial hepatotoxin microcystins (MCs); however, there is a dearth of studies on cyanobacterial neurotoxin anatoxins. In this study, we explored how an anatoxin-producing Cuspidothrix issatschenkoi strain responded to culture with inorganic and organic nitrogen sources in terms of growth and anatoxins production. The results of our study revealed that - alanine could greatly boost cell growth, and was associated with the highest cell productivity, while urea significantly stimulated anatoxin production with the maximum anatoxin yield reaching 25.86 mu g/mg dry weight, which was 1.56-fold higher than that in the control group (BG11). To further understand whether the carbon/nitrogen balance in C. issatschenkoi would affect anatoxin production, we explored growth and toxin production in response to different carbon/nitrogen ratios (C/N). Anatoxin production was mildly promoted when the C/N ratio was within low range, and significantly inhibited when the C/N ratio was within high range, showing approximately a three-fold difference. Furthermore, the transcriptional profile revealed that anaC gene expression was significantly up-regulated over 2-24 h when the C/N ratio was increased, and was significantly down-regulated after 96 h. Overall, our results further enriched the evidence that urea can stimulate cyanotoxin production, and -alanine could boost C. issatschenkoi proliferation, thus providing information for better management of aquatic systems. Moreover, by focusing on the intracellular C/N metabolic balance, this study explained the anatoxin production dynamics in C. issatschenkoi in response to different N sources.
WOS关键词ANABAENA-FLOS-AQUAE ; HOMOANATOXIN-A ; 1ST REPORT ; APHANIZOMENON-ISSATSCHENKOI ; MICROCYSTIN PRODUCTION ; DOG NEUROTOXICOSIS ; NITROGEN-SOURCES ; N-2 FIXATION ; AMINO-ACIDS ; CYANOBACTERIA
资助项目Institute of Hydrobiology ; Chinese Academy of Sciences[Y85Z061601] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2019FBZ06] ; National Natural Science Foundation of China[32071601]
WOS研究方向Food Science & Technology ; Toxicology
语种英语
出版者MDPI
WOS记录号WOS:000593911000001
资助机构Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.ihb.ac.cn/handle/342005/39407]  
专题水生生物研究所_藻类生物学及应用研究中心_期刊论文
通讯作者Gan, Nanqin
作者单位1.Hunan Univ Arts & Sci, Hunan Key Lab Hlth Aquaculture & Prod Proc Dongti, Changde 415000, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714		 Tao, Siyi,Wang, Suqin,Song, Lirong,et al. Understanding the Differences in the Growth and Toxin Production of Anatoxin-Producing Cuspidothrix issatschenkoi Cultured with Inorganic and Organic N Sources from a New Perspective: Carbon/Nitrogen Metabolic Balance[J]. TOXINS,2020,12(11):20.
APA Tao, Siyi,Wang, Suqin,Song, Lirong,&Gan, Nanqin.(2020).Understanding the Differences in the Growth and Toxin Production of Anatoxin-Producing Cuspidothrix issatschenkoi Cultured with Inorganic and Organic N Sources from a New Perspective: Carbon/Nitrogen Metabolic Balance.TOXINS,12(11),20.
MLA Tao, Siyi,et al."Understanding the Differences in the Growth and Toxin Production of Anatoxin-Producing Cuspidothrix issatschenkoi Cultured with Inorganic and Organic N Sources from a New Perspective: Carbon/Nitrogen Metabolic Balance".TOXINS 12.11(2020):20.

入库方式： OAI收割

来源：水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。