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Isolation of axenic cyanobacterium and the promoting effect of associated bacterium on axenic cyanobacterium

文献类型：期刊论文

作者Gao, Suqin6; Kong, Yun2,3,4,5; Yu, Jing6; Miao, Lihong6; Ji, Lipeng5; Song, Lirong1; Zeng, Chi6
刊名BMC BIOTECHNOLOGY
出版日期2020-12-30
卷号20期号:1页码:11
关键词Microcystis aeruginosa Bacterial symbioses Microbial ecology Heterotrophic bacteria Promoting effect
DOI10.1186/s12896-020-00656-5
通讯作者Miao, Lihong(ky020241@hotmail.com)
英文摘要Background Harmful cyanobacterial blooms have attracted wide attention all over the world as they cause water quality deterioration and ecosystem health issues. Microcystis aeruginosa associated with a large number of bacteria is one of the most common and widespread bloom-forming cyanobacteria that secret toxins. These associated bacteria are considered to benefit from organic substrates released by the cyanobacterium. In order to avoid the influence of associated heterotrophic bacteria on the target cyanobacteria for physiological and molecular studies, it is urgent to obtain an axenic M. aeruginosa culture and further investigate the specific interaction between the heterotroph and the cyanobacterium. Results A traditional and reliable method based on solid-liquid alternate cultivation was carried out to purify the xenic cyanobacterium M. aeruginosa FACHB-905. On the basis of 16S rDNA gene sequences, two associated bacteria named strain B905-1 and strain B905-2, were identified as Pannonibacter sp. and Chryseobacterium sp. with a 99 and 97% similarity value, respectively. The axenic M. aeruginosa FACHB-905A (Microcystis 905A) was not able to form colonies on BG(11) agar medium without the addition of strain B905-1, while it grew well in BG(11) liquid medium. Although the presence of B905-1 was not indispensable for the growth of Microcystis 905A, B905-1 had a positive effect on promoting the growth of Microcystis 905A. Conclusions The associated bacteria were eliminated by solid-liquid alternate cultivation method and the axenic Microcystis 905A was successfully purified. The associated bacterium B905-1 has the potentiality to promote the growth of Microcystis 905A. Moreover, the purification technique for cyanobacteria described in this study is potentially applicable to a wider range of unicellular cyanobacteria.
WOS关键词MICROCYSTIS-AERUGINOSA ; HETEROTROPHIC BACTERIA ; PHYLOGENETIC DIVERSITY ; GROWTH ; PURIFICATION ; CULTURE ; WATER ; SPP. ; ESTABLISHMENT ; AGGREGATION
资助项目National High Technology Research and Development Program of China (863 Program)[2013AA102805-04] ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province[2018ZJSHKF06] ; Key Project of Jingzhou Science and Technology[2019EC61-15] ; China Postdoctoral Science Foundation[2016 M591832] ; Natural Science Foundation of Jiangsu Province[BK20150165] ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province[KJ15ZB01]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
出版者BMC
WOS记录号WOS:000594796500001
资助机构National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province
源URL[http://ir.ihb.ac.cn/handle/342005/39413]  
专题水生生物研究所_藻类生物学及应用研究中心_期刊论文
通讯作者Miao, Lihong
作者单位1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Natl Supervis & Inspect Ctr Environm Protect Equi, Yixing Urban Supervis & Inspect Adm Prod Qual, Yixing 214205, Jiangsu, Peoples R China
3.Nanjing Univ, Yixing Acad Environm Protect, Yixing 214200, Jiangsu, Peoples R China
4.Key Lab Water Pollut Control & Environm Safety Zh, Hangzhou 310058, Zhejiang, Peoples R China
5.Yangtze Univ, Coll Resources & Environm, Wuhan 430100, Hubei, Peoples R China
6.Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Gao, Suqin,Kong, Yun,Yu, Jing,et al. Isolation of axenic cyanobacterium and the promoting effect of associated bacterium on axenic cyanobacterium[J]. BMC BIOTECHNOLOGY,2020,20(1):11.
APA Gao, Suqin.,Kong, Yun.,Yu, Jing.,Miao, Lihong.,Ji, Lipeng.,...&Zeng, Chi.(2020).Isolation of axenic cyanobacterium and the promoting effect of associated bacterium on axenic cyanobacterium.BMC BIOTECHNOLOGY,20(1),11.
MLA Gao, Suqin,et al."Isolation of axenic cyanobacterium and the promoting effect of associated bacterium on axenic cyanobacterium".BMC BIOTECHNOLOGY 20.1(2020):11.

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来源：水生生物研究所

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