An identified PfHMGB1 promotes microcystin-LR-induced liver injury of yellow catfish (Pelteobagrus fulvidraco)
文献类型：期刊论文
| 作者 | Wang, Yun2,3; Xiao, Xiaoxue2; Wang, Feijie2; Yang, Zupeng2; Yue, Jingkai2; Shi, Jiale2; Ke, Fei1; Xie, Zhaohui2; Fan, Yanru2 |
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2021 |
| 卷号 | 207页码:11 |
| ISSN号 | 0147-6513 |
| 关键词 | PfHMGB1 MC-LR Hepatotoxicity Liver injury Yellow catfish anti-PfHMGB1 |
| DOI | 10.1016/j.ecoenv.2020.111266 |
| 通讯作者 | Wang, Yun(ywang401@jhun.edu.cn) |
| 英文摘要 | Microcystin-LR (MC-LR) is a potent hepatotoxin that can cause liver inflammation and injury. However, the mode of action of related inflammatory factors is not fully understood. PfHMGB1 is an inflammatory factor induced at the mRNA level in the liver of juvenile yellow catfish (Pelteobagrus fulvidraco) that were intraperi-toneally injected with 50 mu g/kg MC-LR. The PfHMGB1 mRNA level was highest in the liver and muscle among 11 tissues examined. The full-length cDNA sequence of PfHMGB1 was cloned and overexpressed in E. coli, and the purified protein rPfHMGB1 demonstrated DNA binding affinity. Endotoxin-free rPfHMGB1 (6-150 mu g/mL) also showed dose-dependent hepatotoxicity and induced inflammatory gene expression of primary hepatocytes. PfHMGB1 antibody (anti-PfHMGB1) in vitro reduced MC-LR (30 and 50 mu mol/L)-induced hepatotoxicity, suggesting PfHMGB1 is important in the toxic effects of MC-LR. In vivo study showed that MC-LR upregulated PfHMGB1 protein in the liver. The anti-PfHMGB1 blocked its counterpart and reduced ALT/AST activities after MC-LR exposure. Anti-PfHMGB1 partly neutralized MC-LR-induced hepatocyte disorganization, nucleus shrinkage, mitochondria, and rough endoplasmic reticula destruction. These findings suggest that PfHMGB1 promotes MC-LR-induced liver damage in the yellow catfish. HMGB1 may help protect catfish against widespread microcystin pollution. |
| WOS关键词 | GROUP BOX 1 ; INDUCED HEPATOCYTE APOPTOSIS ; DNA-BINDING ; TIGHT JUNCTIONS ; IN-VIVO ; HMGB1 ; EXPRESSION ; PROTEIN ; INFLAMMATION ; RESPONSES |
| 资助项目 | National Natural Science Foundation of China[31702388] |
| WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000592451000008 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/39401]  |
| 专题 | 水生生物研究所_其他_期刊论文 |
| 通讯作者 | Wang, Yun |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Henan Univ Urban Construct, Henan Prov Key Lab Water Pollut Control & Rehabil, Pingdingshan 467036, Peoples R China 3.Jianghan Univ, Inst Environm & Hlth, Hubei Key Lab Environm & Hlth Effects Persistent, Wuhan 430056, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yun,Xiao, Xiaoxue,Wang, Feijie,et al. An identified PfHMGB1 promotes microcystin-LR-induced liver injury of yellow catfish (Pelteobagrus fulvidraco)[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,207:11. |
| APA | Wang, Yun.,Xiao, Xiaoxue.,Wang, Feijie.,Yang, Zupeng.,Yue, Jingkai.,...&Fan, Yanru.(2021).An identified PfHMGB1 promotes microcystin-LR-induced liver injury of yellow catfish (Pelteobagrus fulvidraco).ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,207,11. |
| MLA | Wang, Yun,et al."An identified PfHMGB1 promotes microcystin-LR-induced liver injury of yellow catfish (Pelteobagrus fulvidraco)".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 207(2021):11. |
入库方式： OAI收割
来源：水生生物研究所
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