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Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors

文献类型：期刊论文

作者He, Mudan1,2; Zhang, Ru1,2; Jiao, Shengbo1,2; Zhang, Fenghua1,2; Ye, Ding1,2; Wang, Houpeng1,2; Sun, Yonghua1,2
刊名PLOS BIOLOGY
出版日期2020-07-01
卷号18期号:7页码:32
ISSN号1544-9173
DOI10.1371/journal.pbio.3000561
通讯作者Sun, Yonghua(yhsun@ihb.ac.cn)
英文摘要Maternal beta-catenin activity is essential and critical for dorsal induction and its dorsal activation has been thoroughly studied. However, how the maternal beta-catenin activity is suppressed in the nondorsal cells remains poorly understood. Nanog is known to play a central role for maintenance of the pluripotency and maternal -zygotic transition (MZT). Here, we reveal a novel role of Nanog as a strong repressor of maternal beta-catenin signaling to safeguard the embryo against hyperactivation of maternal beta-catenin activity and hyperdorsalization. In zebrafish, knockdown of nanog at different levels led to either posteriorization or dorsalization, mimicking zygotic or maternal activation of Wnt/beta-catenin activities, and the maternal zygotic mutant of nanog (MZnanog) showed strong activation of maternal beta-catenin activity and hyperdorsalization. Although a constitutive activator-type Nanog (Vp16-Nanog, lacking the N terminal) perfectly rescued the MZT defects of MZnanog, it did not rescue the phenotypes resulting from beta-catenin signaling activation. Mechanistically, the N terminal of Nanog directly interacts with T-cell factor (TCF) and interferes with the binding of beta-catenin to TCF, thereby attenuating the transcriptional activity of beta-catenin. Therefore, our study establishes a novel role for Nanog in repressing maternal beta-catenin activity and demonstrates a transcriptional switch between beta-catenin/TCF and Nanog/TCF complexes, which safeguards the embryo from global activation of maternal beta-catenin activity.
WOS关键词WNT SIGNALING PATHWAY ; EMBRYONIC-DEVELOPMENT ; TRANSCRIPTION FACTORS ; DISTINCT ROLES ; AXIS FORMATION ; ZEBRAFISH ; ORGANIZER ; GROUCHO ; MESODERM ; GENES
资助项目National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31671501] ; National Natural Science Foundation of China[31702323] ; National key R&D Program of China[2018YFA0801000] ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology[2019FBZ05]
WOS研究方向Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
语种英语
WOS记录号WOS:000596578100001
出版者PUBLIC LIBRARY SCIENCE
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology
源URL[http://ir.ihb.ac.cn/handle/342005/39382]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Sun, Yonghua
作者单位1.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
推荐引用方式
GB/T 7714		 He, Mudan,Zhang, Ru,Jiao, Shengbo,et al. Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors[J]. PLOS BIOLOGY,2020,18(7):32.
APA He, Mudan.,Zhang, Ru.,Jiao, Shengbo.,Zhang, Fenghua.,Ye, Ding.,...&Sun, Yonghua.(2020).Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors.PLOS BIOLOGY,18(7),32.
MLA He, Mudan,et al."Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors".PLOS BIOLOGY 18.7(2020):32.

入库方式： OAI收割

来源：水生生物研究所

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