中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 水生生物研究所
  3. 鱼类生物学及渔业生物技术研究中心
  4. 期刊论文
Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors

文献类型：期刊论文

作者He, Mudan1,2; Zhang, Ru1,2; Jiao, Shengbo1,2; Zhang, Fenghua1,2; Ye, Ding1,2; Wang, Houpeng1,2; Sun, Yonghua1,2
刊名PLOS BIOLOGY
出版日期2020-07-01
卷号18期号:7页码:32
ISSN号1544-9173
DOI10.1371/journal.pbio.3000561
通讯作者Sun, Yonghua(yhsun@ihb.ac.cn)
英文摘要Maternal beta-catenin activity is essential and critical for dorsal induction and its dorsal activation has been thoroughly studied. However, how the maternal beta-catenin activity is suppressed in the nondorsal cells remains poorly understood. Nanog is known to play a central role for maintenance of the pluripotency and maternal -zygotic transition (MZT). Here, we reveal a novel role of Nanog as a strong repressor of maternal beta-catenin signaling to safeguard the embryo against hyperactivation of maternal beta-catenin activity and hyperdorsalization. In zebrafish, knockdown of nanog at different levels led to either posteriorization or dorsalization, mimicking zygotic or maternal activation of Wnt/beta-catenin activities, and the maternal zygotic mutant of nanog (MZnanog) showed strong activation of maternal beta-catenin activity and hyperdorsalization. Although a constitutive activator-type Nanog (Vp16-Nanog, lacking the N terminal) perfectly rescued the MZT defects of MZnanog, it did not rescue the phenotypes resulting from beta-catenin signaling activation. Mechanistically, the N terminal of Nanog directly interacts with T-cell factor (TCF) and interferes with the binding of beta-catenin to TCF, thereby attenuating the transcriptional activity of beta-catenin. Therefore, our study establishes a novel role for Nanog in repressing maternal beta-catenin activity and demonstrates a transcriptional switch between beta-catenin/TCF and Nanog/TCF complexes, which safeguards the embryo from global activation of maternal beta-catenin activity.
WOS关键词WNT SIGNALING PATHWAY ; EMBRYONIC-DEVELOPMENT ; TRANSCRIPTION FACTORS ; DISTINCT ROLES ; AXIS FORMATION ; ZEBRAFISH ; ORGANIZER ; GROUCHO ; MESODERM ; GENES
资助项目National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31671501] ; National Natural Science Foundation of China[31702323] ; National key R&D Program of China[2018YFA0801000] ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology[2019FBZ05]
WOS研究方向Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
语种英语
出版者PUBLIC LIBRARY SCIENCE
WOS记录号WOS:000596578100001
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology
源URL[http://ir.ihb.ac.cn/handle/342005/39382]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Sun, Yonghua
作者单位1.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
推荐引用方式
GB/T 7714		 He, Mudan,Zhang, Ru,Jiao, Shengbo,et al. Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors[J]. PLOS BIOLOGY,2020,18(7):32.
APA He, Mudan.,Zhang, Ru.,Jiao, Shengbo.,Zhang, Fenghua.,Ye, Ding.,...&Sun, Yonghua.(2020).Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors.PLOS BIOLOGY,18(7),32.
MLA He, Mudan,et al."Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors".PLOS BIOLOGY 18.7(2020):32.

入库方式： OAI收割

来源：水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。