Currently, most rivers worldwide have been intensively impounded. River damming becomes a big problem, not only in inducing the physical obstruction between upstream and downstream, but also in destroying the natural continuity of river. But the discontinuity of water quality was often neglected, which presents a challenge to traditional river geochemistry research. To understand the changes in basic chemistry of water upstream and downstream of the dam, we investigated the Miaotiao River reservoirs in series in the Wujiang River Basin, and the Hongjiadu, Dongfeng Reservoir on the upper reaches of the Wujiang River. Chemical weathering rates were calculated using the water chemistry data of the reservoir surface and downstream of the dam, in each reservoir, respectively. The results showed that the difference between the chemical weathering rates calculated from reservoir surface water and water downstream of the dam was greater in reservoirs with a longer water retention time. In Hongjiadu Reservoir with the longest water retention time among the studied reservoirs, this difference reaches 9%. As a result, the influence of river damming, especially the influence of reservoirs in series, should be taken into account when calculating the chemical weathering rate of a river basin.