Construction of a novel bispecific fusion protein to enhance targeting for pancreatic cancer imaging
文献类型:期刊论文
作者 | Wang, Qian3,5; Yan, Hao1,4; Wang, Zihua7; Li, Zhangfu6; Li, Dan6; Li, Zheng2; Wang, Kun5; Tian, Jie5; Zhao, Xinming3 |
刊名 | BIOMATERIALS |
出版日期 | 2020-10-01 |
期号 | 255页码:120161 |
ISSN号 | 0142-9612 |
关键词 | Bispecific targeting Fusion protein Antibody Molecular imaging Pancreatic cancer |
DOI | 10.1016/j.biomaterials.2020.120161 |
英文摘要 | Early detection and diagnosis are the most important endeavors for reducing associated morbidity and mortality of pancreatic ductal adenocarcinoma (PDAC). Developing molecular imaging probes that can specifically and effectively target cancer-associated biological pathways is one of the key points for sensitive and accurate diagnosis for PDAC. Herein, a small-sized, bispecific fusion protein constructed by genetic fusion of different binding domains of antibodies, termed Bi50, with enhanced targeting effect for PDAC is reported. Bi50 has excellent bispecific targeting for vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) simultaneously in vitro and in vivo. Additionally, Bi50 shows increased intratumoral permeability and enrichment characteristics in the tumor than the control protein, which is constructed directly connecting two individual Fabs. Moreover, Bi50 can not only target areas rich in vasculature but also bind with affinity to tumor parenchymal cells, achieving "multilevel" targeting effect. Our work demonstrates that the bispecific fusion protein Bi50 has great potential as an efficient, targeted molecular imaging probe. |
WOS关键词 | EGFR ; INHIBITORS ; SURVIVAL ; EFFICACY ; BARRIERS ; SURFACE ; VEGFR |
资助项目 | National Natural Science Foundation of China[81,901,813] ; National Natural Science Foundation of China[81,671,757] ; National Natural Science Foundation of China[81,527,805] ; National Natural Science Foundation of China[81,801,766] ; National Natural Science Foundation of China[61,671,449] ; National Key Research and Development Program of China[2017YFA0205200] ; National Key Research and Development Program of China[2016YFA0201401] ; National Key Research and Development Program of China[2106YFC0103702] ; CAMS Innovation Fund for Medical Sciences[2016-I2M-1-001] |
WOS研究方向 | Engineering ; Materials Science |
语种 | 英语 |
出版者 | ELSEVIER SCI LTD |
WOS记录号 | WOS:000555693800016 |
资助机构 | National Natural Science Foundation of China ; National Key Research and Development Program of China ; CAMS Innovation Fund for Medical Sciences |
源URL | [http://ir.ia.ac.cn/handle/173211/40376] |
专题 | 自动化研究所_中国科学院分子影像重点实验室 |
通讯作者 | Wang, Kun; Tian, Jie; Zhao, Xinming |
作者单位 | 1.Harvard Med Sch, 50 Blossom St, Boston, MA 02114 USA 2.Yi Chuang Inst Biotechnol Ind, Beijing 101111, Peoples R China 3.Chinese Acad Med Sci & Peking Union Med Coll, Dept Diagnost Imaging, Natl Canc Ctr, Canc Hosp, Beijing 100021, Peoples R China 4.Massachusetts Gen Hosp, Wellman Ctr Photomed, 50 Blossom St, Boston, MA 02114 USA 5.Chinese Acad Sci, CAS Key Lab Mol Imaging, Inst Automat, Beijing 100190, Peoples R China 6.Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Mol Oncol, Natl Canc Ctr, Canc Hosp, Beijing 100021, Peoples R China 7.Fujian Med Univ, Sch Basic Med Sci, Fuzhou 350108, Fujian, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Qian,Yan, Hao,Wang, Zihua,et al. Construction of a novel bispecific fusion protein to enhance targeting for pancreatic cancer imaging[J]. BIOMATERIALS,2020(255):120161. |
APA | Wang, Qian.,Yan, Hao.,Wang, Zihua.,Li, Zhangfu.,Li, Dan.,...&Zhao, Xinming.(2020).Construction of a novel bispecific fusion protein to enhance targeting for pancreatic cancer imaging.BIOMATERIALS(255),120161. |
MLA | Wang, Qian,et al."Construction of a novel bispecific fusion protein to enhance targeting for pancreatic cancer imaging".BIOMATERIALS .255(2020):120161. |
入库方式: OAI收割
来源:自动化研究所
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