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Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics

文献类型：期刊论文

作者Wang Sheng-Xiong6,7; Chen Zhen-Qiang1; Han Hua4; Guo Ai-Ke2,3,5,6,7
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
出版日期2020-10-01
卷号47期号:10页码:1090-1096
关键词brain connectomics volume electron microscopy sample preparation
ISSN号1000-3282
DOI10.16476/j.pibb.2020.0011
通讯作者Han Hua() ; Guo Ai-Ke(akguo@ion.ac.cn)
英文摘要For micro-brain connectomic research, volume electron microscopy is a main method in which it plays an importatnt role for the appliaction of automated tape-collecting ultramicrotome (ATUM) system and arrayimaging with scanning electron microscopy together. ATUM system enables collecting a large amount of special sections to be imaged with SEM to form ahuge dataset. With regard tothe nature of non-vacuum sectoningand sample reserving. we modified and improved the sample preparatio process includingresinrecipe, HRP labelling and post-staing method i orderto reduce folding rate of sample sections and make it possible for identifyig certain types of neurons in high-contrast imaging dataset, thus obtainig dataset of high quality for connectomic research.
资助项目Strategic Priority Research Program of Chinese Academy of Science[XDB32010100]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
语种英语
WOS记录号WOS:000583458700007
出版者CHINESE ACAD SCIENCES, INST BIOPHYSICS
资助机构Strategic Priority Research Program of Chinese Academy of Science
源URL[http://ir.ia.ac.cn/handle/173211/41748]  
专题类脑智能研究中心_微观重建与智能分析
通讯作者Han Hua; Guo Ai-Ke
作者单位1.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Luwan Branch, Shanghai 200020, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China
3.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, State Key Lab Neurosci, Inst Neurosci, Shanghai 200031, Peoples R China
4.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China
5.Shanghai Ctr Brain Sci & Inspired Intelligence Te, Shanghai 201210, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
7.Chinese Acad Sci, State Key Lab Brain & Cognit Sci, Inst Biophys, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714		 Wang Sheng-Xiong,Chen Zhen-Qiang,Han Hua,et al. Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2020,47(10):1090-1096.
APA Wang Sheng-Xiong,Chen Zhen-Qiang,Han Hua,&Guo Ai-Ke.(2020).Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,47(10),1090-1096.
MLA Wang Sheng-Xiong,et al."Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 47.10(2020):1090-1096.

入库方式： OAI收割

来源：自动化研究所

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