The first visualization of chemotherapy-induced tumor apoptosis via magnetic particle imaging in a mouse model
文献类型：期刊论文
| 作者 | Liang, Xin1,2; Wang, Kun3 ; Du, Jiangfeng1; Tian, Jie3; Zhang, Hui1,2
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| 刊名 | PHYSICS IN MEDICINE AND BIOLOGY
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| 出版日期 | 2020-10-07 |
| 卷号 | 65期号:19页码:11 |
| 关键词 | magnetic particle imaging apoptosis nanoparticle preclinical mice tumor |
| ISSN号 | 0031-9155 |
| DOI | 10.1088/1361-6560/abad7c |
| 通讯作者 | Tian, Jie(jie.tian@ia.ac.cn) ; Zhang, Hui(zhanghui_mr@163.com) |
| 英文摘要 | Imaging technologies that allow non-radiative visualization and quantification of apoptosis have a great potential for assessing therapy response, early diagnosis, and disease monitoring. Magnetic particle imaging (MPI), the direct imaging of magnetic nanoparticles as positive contrast agent and sole signal source, enables high image contrast (no tissue background signal), potential high sensitivity, and quantifiable signal intensity. These properties confer a great potential for application to tumor apoptosis monitoring. In this study, a simple and robust method was used to conjugate Alexa Fluor 647-AnnexinV (AF647-Anx), which can avidly bind to apoptotic cells, to superparamagnetic iron oxide (SPIO) nanoparticles, termed AF647-Anx-SPIO, which serves as an MPI-detectable tracer. Based on this apoptosis-specific tracer, MPI can accurately and unambiguously detect and quantify apoptotic tumor cells. AF647-Anx-SPIO showed relatively high affinity for apoptotic cells, and differences in binding between treated (apoptotic rate 67.21% +/- 1.36%) and untreated (apoptotic rate 10.12 +/- 0.11%) cells could be detected by MPIin vitro(P< 0.05). Moreover, the imaging signal was almost proportional to the number of apoptotic cells determined using an MPI scanner (R-2= 0.99). There was a greater accumulation of AF647-Anx-SPIO in tumors of drug-treated animals than in tumors of untreated animals (P< 0.05), and the difference could be detected by MPIex vivo, while forin vivoimaging, no MPI imaging signal was detected in either group. Overall, this preliminary study demonstrates that MPI could be a potential imaging modality for tumor apoptosis imaging. |
| WOS关键词 | IN-VIVO ; ANNEXIN-V ; HIGH-PERFORMANCE ; NANOPARTICLES ; CANCER ; PHOSPHATIDYLSERINE ; BIODISTRIBUTION ; EXPRESSION ; CLEARANCE ; PATHWAYS |
| 资助项目 | Ministry of Science and Technology of China[2017YFA0205200] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[61671449] ; Chinese Academy of Sciences[GJJSTD20170004] ; Chinese Academy of Sciences[KFJ-STS-ZDTP-059] ; Chinese Academy of Sciences[YJKYYQ20180048] ; Chinese Academy of Sciences[XDB32030200] ; Chinese Academy of Sciences[QYZDJ-SSW-JSC005] |
| WOS研究方向 | Engineering ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000575512700001 |
| 出版者 | IOP PUBLISHING LTD |
| 资助机构 | Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences |
| 源URL | [http://ir.ia.ac.cn/handle/173211/42028]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 通讯作者 | Tian, Jie; Zhang, Hui |
| 作者单位 | 1.Shanxi Med Univ, Coll Med Imaging, Taiyuan, Shanxi, Peoples R China 2.Shanxi Med Univ, Clin Med Coll 1, Dept Radiol, Taiyuan, Shanxi, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Xin,Wang, Kun,Du, Jiangfeng,et al. The first visualization of chemotherapy-induced tumor apoptosis via magnetic particle imaging in a mouse model[J]. PHYSICS IN MEDICINE AND BIOLOGY,2020,65(19):11. |
| APA | Liang, Xin,Wang, Kun,Du, Jiangfeng,Tian, Jie,&Zhang, Hui.(2020).The first visualization of chemotherapy-induced tumor apoptosis via magnetic particle imaging in a mouse model.PHYSICS IN MEDICINE AND BIOLOGY,65(19),11. |
| MLA | Liang, Xin,et al."The first visualization of chemotherapy-induced tumor apoptosis via magnetic particle imaging in a mouse model".PHYSICS IN MEDICINE AND BIOLOGY 65.19(2020):11. |
入库方式： OAI收割
来源：自动化研究所
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