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Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell-Based Medicine

文献类型：期刊论文

作者Padhiar, Arshad Ahmed3,4; Faqeer, Abdullah5; Sun, Shimin6; Hamid, Md Rana3,4; Liao, Jinqi3,4; Yan Zhou7; Ahmmed, Bulbul3,4; Qin, Xialing5; Tie Changjun8; Gao, Shuping9
刊名SMALL METHODS
出版日期2020-10-08
页码13
ISSN号2366-9608
关键词hepatic transporters magnetic resonance imaging mesenchymal stem cells near-infrared region non-invasive multimodality imaging
DOI10.1002/smtd.202000491
通讯作者Chen Zhang(czhang@ccmu.edu.cn) ; Zhou, Guangqian(gqzhou@szu.edu.cn)
英文摘要Hepatic transporters facilitate the uptake of xenobiotic compounds and confer immense potential as multimodality reporters, as they can uptake multitude of bioluminescent, fluorescence, near Infra-red (NIR), and magnetic resonance imaging (MRI) compounds. Here, it is aimed to identify the prospects of three human hepatocyte membrane proteins, organic anion transporters (OATP) 1B1, OATP1B3, and sodium-taurocholate cotransporting polypeptide in tracing the transplanted cells dynamics, by the uptake of clinically approved MRI compound, gadolinium ethoxybenzyl-diethylenetriaminepentaacetic acid (GD-EOB DTPA) or NIR fluorescent dye, Indocyanine Green (ICG). First, OATP1B1 is introduced as new MRI reporter which is the human orthologue of previously established rodent (Oatp1a1) reporter. Comparative sequential assays by assimilating MR image parameters reveal OATP1B3 as superior reporter on T1-weighted images and display highest contrast enhancement reported to-date when using a clinical 3T MR scanner (approximate to 21-fold in vitro; approximate to 8-fold in vivo). Stably expressing these hepatic transporters have no effect on human adipose derived mesenchymal stem cells (hADSCs) characteristics. However, only OATP1B3 able to trace as few as 2 x 10(5)hADSCs intramuscular xenograft survival time on NIR and 3T MRI. These data suggest that OATP1B3 is relatively a robust Gd-EOB-DTPA/ICG-dependent multimodality reporter in visualizing dynamic processes for cell-based therapies.
WOS关键词INDOCYANINE GREEN ; EXPRESSION ; FLUORESCENCE
资助项目National Key R&D Program of China[2017FA105202] ; Shenzhen Science and Technology Innovation Committee[JCYJ20170818094109846] ; Shenzhen Science and Technology Innovation Committee[GJHZ20180928155604617] ; Shenzhen Science and Technology Innovation Committee[JCYJ20170307155449972] ; Natural Science Foundation[81772333] ; Natural Science Foundation[81774053]
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science
语种英语
出版者WILEY-V C H VERLAG GMBH
WOS记录号WOS:000575956200001
资助机构National Key R&D Program of China ; Shenzhen Science and Technology Innovation Committee ; Natural Science Foundation
源URL[http://ir.ia.ac.cn/handle/173211/42068]  
专题自动化研究所_脑网络组研究中心
通讯作者Chen Zhang; Zhou, Guangqian
作者单位1.Chinese Acad Sci, Brainnetome Ctr, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Natl Lab Pattern Recognit, Beijing 100190, Peoples R China
3.Shenzhen Univ, Hlth Sci Ctr, Shenzhen Key Lab Antiaging & Regenerat Med, Dept Med Cell Biol & Genet,Guangdong Key Lab Geno, Shenzhen 518060, Peoples R China
4.Shenzhen Univ, Hlth Sci Ctr, Shenzhen Engn Lab Regenerat Technol Orthopaed Dis, Shenzhen 518060, Peoples R China
5.Shenzhen Univ, Sch Biomed Engn, Hlth Sci Ctr, Shenzhen 518060, Peoples R China
6.Shenzhen Univ, Hlth Sci Ctr, Guangdong Key Lab Genome Stabil & Human Dis Preve, Shenzhen, Peoples R China
7.Lungene Technol, 8606,Yinxing Sci Bldg, Shenzhen 510086, Peoples R China
8.Chinese Acad Sci, Paul C Lauterbur Res Ctr Biomed Imaging, Shenzhen Inst Adv Technol, Shenzhen, Peoples R China
9.Peking Univ, Sch Life Sci, PKU IDG McGovern Inst Brain Res, State Key Lab Membrane Biol, Beijing 100871, Peoples R China
10.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Inst Automat, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Padhiar, Arshad Ahmed,Faqeer, Abdullah,Sun, Shimin,et al. Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell-Based Medicine[J]. SMALL METHODS,2020:13.
APA Padhiar, Arshad Ahmed.,Faqeer, Abdullah.,Sun, Shimin.,Hamid, Md Rana.,Liao, Jinqi.,...&Zhou, Guangqian.(2020).Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell-Based Medicine.SMALL METHODS,13.
MLA Padhiar, Arshad Ahmed,et al."Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell-Based Medicine".SMALL METHODS (2020):13.

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来源：自动化研究所

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