A label-free quantification method for measuring graphene oxide in biological samples
文献类型：期刊论文
| 作者 | Xin, Yan2; Wan, Bin2 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2019-11-04 |
| 卷号 | 1079页码:103-110 |
| 关键词 | Graphene oxide Gel electrophoresis Exposure quantification Cell uptake |
| ISSN号 | 0003-2670 |
| 英文摘要 | Characterization of carbonaceous nanomaterials (CNMs) exposure is a key step and of great importance towards a better understanding of their toxicity and underlying mechanisms. However, it has been bottlenecked for lack of valid methods capable of quantifying cell-associated CNMs. Here, we developed a new economical and convenient method based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) that could accumulate graphene oxide (GO) at the interface between the loading well and the gel. The sharp black band formed there can be digitalized and the intensity quantified, which was proportional to the amount of GO loaded onto the gel. The method has a detection limit of 84.1 ng. We showed that the amount of GO in three different cell models, mouse macrophage cells (Raw264.7), human epithelial cells (A549) and mouse mesenchymal stem cells (MSC), could be accurately quantified by this assay, with the uptake rates decreasing in the order of MSC > Raw264.7 > A549. The results were consistent with the fluorescent imaging on cells exposed to fluorescence-labeled GO and TEM examination on ultrathin cell sections. The surprisingly highest uptake rate of MSC might be due to their abundant intracellular vesicles, which deserves further investigation. The novel method provides a complementary quantitative tool to the use of radioactive markers and fluorescent labeling of carbon nanomaterials and may facilitate the toxicological studies on carbon nanomaterials. (C) 2019 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42493]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xin, Yan,Wan, Bin. A label-free quantification method for measuring graphene oxide in biological samples[J]. ANALYTICA CHIMICA ACTA,2019,1079:103-110. |
| APA | Xin, Yan,&Wan, Bin.(2019).A label-free quantification method for measuring graphene oxide in biological samples.ANALYTICA CHIMICA ACTA,1079,103-110. |
| MLA | Xin, Yan,et al."A label-free quantification method for measuring graphene oxide in biological samples".ANALYTICA CHIMICA ACTA 1079(2019):103-110. |
入库方式： OAI收割
来源：生态环境研究中心
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