A selective adsorption-based separation of low-mass molecules from biological samples towards high-throughput mass spectrometry analysis in a single drop of human whole blood
文献类型：期刊论文
| 作者 | Wang, Jing2,4 ; Liang, Yong4; Mao, Yuxiang2; Liu, Qian3,4,5; Jiang, Guibin3,5
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| 刊名 | TALANTA
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| 出版日期 | 2019-09-01 |
| 卷号 | 202页码:237-243 |
| 关键词 | Ordered mesoporous carbon Adsorbent Sample preparation MALDI-TOF MS Mass spectrometry Single-drop whole blood |
| ISSN号 | 0039-9140 |
| 英文摘要 | In this work, a newly designed multifunctional adsorbent material, octadecyl-modified ordered mesoporous carbon (C-18-CMK-8), was synthesized and employed for simultaneous analysis of multiple small molecules by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The C-18-CMK-8 was facilely prepared by one-step chemical binding of octadecyltrimethoxysilane to an ordered mesoporous carbon CMK-8. The nanosized pore structure of C-18-CMK-8 can selectively enrich low-mass molecules while excluding interferences from large molecules in whole blood; meanwhile, the C-18 chain can greatly enhance the affinity for a wide range of small molecules. Then, the C-18-CMK-8-based solid-phase extraction (SPE) combined with MALDI-TOF MS measurement was applied in rapid and high-throughput screening of six doping agents (triamterene, trenbolone, testosterone, methyltestosterone, clenbuterol, and strychnine)) in single-drop human whole blood samples. The method showed high analytical sensitivity (detection limit 0.05-0.1 ng mL(-1)), good recoveries (67.2-114.3%), minimal sample requirement (similar to 20 mu L), and robust anti-interference ability. With distinct advantages such as high throughput, rapidness, minimal sample requirement, and high sensitivity, this study not only offers a versatile enrichment material for complex sample preparation, but also demonstrates a promising tool for minimal whole blood analysis. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42499]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 3.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China 4.Jianghan Univ, Hubei Key Lab Environm & Hlth Effects Persistent, Wuhan 430056, Hubei, Peoples R China 5.Henan Polytech Univ, Inst Resources & Environm, Jiaozuo 454003, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jing,Liang, Yong,Mao, Yuxiang,et al. A selective adsorption-based separation of low-mass molecules from biological samples towards high-throughput mass spectrometry analysis in a single drop of human whole blood[J]. TALANTA,2019,202:237-243. |
| APA | Wang, Jing,Liang, Yong,Mao, Yuxiang,Liu, Qian,&Jiang, Guibin.(2019).A selective adsorption-based separation of low-mass molecules from biological samples towards high-throughput mass spectrometry analysis in a single drop of human whole blood.TALANTA,202,237-243. |
| MLA | Wang, Jing,et al."A selective adsorption-based separation of low-mass molecules from biological samples towards high-throughput mass spectrometry analysis in a single drop of human whole blood".TALANTA 202(2019):237-243. |
入库方式： OAI收割
来源：生态环境研究中心
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