Characterization of the Aryl Hydrocarbon Receptor (AhR) Pathway in Anabas testudineus and Mechanistic Exploration of the Reduced Sensitivity of AhR2a
文献类型：期刊论文
| 作者 | Zhang, Wanglong1; Xie, Heidi Qunhui1; Li, Yunping1; Zou, Xianghui2; Xu, Li1; Ma, Dan1; Li, Jiao1; Ma, Yongchao1; Jin, Tao3,4; Hahn, Mark E. |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2019-11-05 |
| 卷号 | 53期号:21页码:12803-12811 |
| ISSN号 | 0013-936X |
| 英文摘要 | Field investigations have revealed the ability of the climbing perch Anabas testudineus to survive in highly contaminated water bodies. The aryl hydrocarbon receptor (AhR) pathway is vital in mediating the toxicity of aromatic hydrocarbon contaminants, and genotypic variation in the AhR can confer resistance to these contaminants. Thus, we characterized the AhR pathway in A. testudineus in order to understand the mechanism(s) underlying the resistance of this species to contaminants and to broaden current knowledge on teleost AhR. In A. testudineus, four AhRs, two AhR nuclear translocators (ARNTs), and one AhR repressor (AhRR) were found. Transient transfection assays revealed that AhR1a, AhR1b, and AhR2b were functional, whereas AhR2a was poorly activated by the potent agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Two ARNTs (partner of AhR) and one AhRR (repressor of AhR) all were functional with each of the active AhR As a major form, the insensitivity of AhR2a might serve as a potential mechanism for A. testudineus' reduced sensitivity to severe contamination. We explored the key residues that may account for AhR2a's insensitivity in silico and then functionally validated them in vitro. Two sites (VCS322-324, M-370) in its ligand-binding domain (LBD) were proved critical for its sensitivity to TCDD. This systematic exploration of the AhR pathway showed that most members have maintained their traditional functions as expected, whereas a nonfunctionalization event has occurred for AhR2a. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42522]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hanshan Normal Univ, Sch Food Engn & Biotechnol, Chaozhou 521041, Peoples R China 4.China Natl Genebank Shenzhen, BGI Shenzhen, Shenzhen 518083, Peoples R China 5.BGI Qingdao, Qingdao 266510, Shandong, Peoples R China 6.Hahn, Mark E.] Woods Hole Oceanog Inst WHOI, Dept Biol, Woods Hole, MA 02543 USA 7.Hahn, Mark E.] Boston Univ, Superfund Res Program, Boston, MA 02118 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Wanglong,Xie, Heidi Qunhui,Li, Yunping,et al. Characterization of the Aryl Hydrocarbon Receptor (AhR) Pathway in Anabas testudineus and Mechanistic Exploration of the Reduced Sensitivity of AhR2a[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2019,53(21):12803-12811. |
| APA | Zhang, Wanglong.,Xie, Heidi Qunhui.,Li, Yunping.,Zou, Xianghui.,Xu, Li.,...&Zhao, Bin.(2019).Characterization of the Aryl Hydrocarbon Receptor (AhR) Pathway in Anabas testudineus and Mechanistic Exploration of the Reduced Sensitivity of AhR2a.ENVIRONMENTAL SCIENCE & TECHNOLOGY,53(21),12803-12811. |
| MLA | Zhang, Wanglong,et al."Characterization of the Aryl Hydrocarbon Receptor (AhR) Pathway in Anabas testudineus and Mechanistic Exploration of the Reduced Sensitivity of AhR2a".ENVIRONMENTAL SCIENCE & TECHNOLOGY 53.21(2019):12803-12811. |
入库方式： OAI收割
来源：生态环境研究中心
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