Diastereoisomer-specific neurotoxicity of hexabromocyclododecane in human SH-SY5Y neuroblastoma cells
文献类型：期刊论文
| 作者 | Shi, Xiaoli2; Zha, Jinmiao2; Wen, Bei; Zhang, Shuzhen2 |
| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| 出版日期 | 2019-10-10 |
| 卷号 | 686页码:893-902 |
| 关键词 | Hexabromocyclododecane diastereoisomers SH-SY5Y cells Neurotoxidty Apoptosis Oxidative stress |
| ISSN号 | 0048-9697 |
| 英文摘要 | Hexabromocyclododecane (HBCD) is a widely applied brominated flame retardant (BFR) and is regarded as a persistent organic pollutant. It has been found in human tissues and has the potential to cause neurological disorders. However, our understanding of HBCD neurotoxicity at the diastereoisomer level remains lacking. Here, we investigated the neurotoxicity of three HBCD diastereoisomers, i.e., alpha-, beta-, and gamma-HBCD, in SH-SY5Y human neuroblastoma cells. Results showed that the HBCD diastereoisomers decreased cell viability, increased lactate dehydrogenase (LDH) release, and impaired cytoskeleton development. Typical morphological features and apoptosis rates showed that the HBCD diastereoisomers induced SH-SY5Y cell apoptosis. The expression levels of several cell apoptosis-related genes and proteins, including Bax, caspase-3, caspase-9, cytochrome c, Bcl-2, and X-linked inhibitor of apoptosis (XIAP), as well as the cell cycle arrest, DNA damage, adenosine triphosphate (ATP) consumption, reactive oxygen species (ROS) levels, and intracellular calcium ion (Ca2+) levels, were examined. Results showed that the HBCD diastereoisomer neurotoxicity was ranked beta-HBCD> gamma-HBCD > alpha-HBCD. The cell apoptosis and caspase expression levels of the three HBCD diastereoisomers followed the same order, suggesting that caspase-dependent apoptosis may be one mechanism responsible for the structure-selective HBCD diastereoisomer neurotoxicity. The levels of intracellular Ca2+ and ROS increased significantly. The ROS levels were ordered beta-HBCD > gamma-HBCD > alpha-HBCD. whereas those of intracellular Ca2+ were gamma-HBCD > beta-HBCD > alpha-HBCD. Thus, ROS may be a key factor regulating the neurotoxicity of HBCD diastereoisomers. To the best of our knowledge, this is the first study to report on the diastereoisomer-specific toxicity of HBCD in human neural cells and on the possible mechanisms responsible for the selective neurotoxicity of HBCD diastereoisomers. (C) 2019 Published by Elsevier B.V. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42528]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Xiaoli,Zha, Jinmiao,Wen, Bei,et al. Diastereoisomer-specific neurotoxicity of hexabromocyclododecane in human SH-SY5Y neuroblastoma cells[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,686:893-902. |
| APA | Shi, Xiaoli,Zha, Jinmiao,Wen, Bei,&Zhang, Shuzhen.(2019).Diastereoisomer-specific neurotoxicity of hexabromocyclododecane in human SH-SY5Y neuroblastoma cells.SCIENCE OF THE TOTAL ENVIRONMENT,686,893-902. |
| MLA | Shi, Xiaoli,et al."Diastereoisomer-specific neurotoxicity of hexabromocyclododecane in human SH-SY5Y neuroblastoma cells".SCIENCE OF THE TOTAL ENVIRONMENT 686(2019):893-902. |
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来源：生态环境研究中心
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