Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on acetylcholinesterase during myogenic differentiation of contractile rat primary skeletal muscle cells
文献类型：期刊论文
| 作者 | Luo, Yali3; Xie, Heidi Qunhui3; Chen, Yangsheng3; Xia, Yingjie; Sha, Rui3; Liu, Yiyun3; Ma, Yongchao3; Xu, Tong3 ; Xu, Li3; Tsim, Karl Wah-Keung
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| 刊名 | CHEMICO-BIOLOGICAL INTERACTIONS
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| 出版日期 | 2019-08-01 |
| 卷号 | 308页码:164-169 |
| 关键词 | Dioxin Acetylcholinesterase Muscle contraction Aryl hydrocarbon receptor Primary skeletal muscle cell |
| ISSN号 | 0009-2797 |
| 英文摘要 | Emerging data indicate that prenatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) could interfere with myogenic differentiation in vivo. Acetylcholinesterase (EC3.1.1.7; AChE), an enzyme critical for cholinergic neurotransmission, is abundantly expressed in neurons and mature myotubes, and we recently found that muscle AChE expression was suppressed in parallel with the inhibition of myogenic differentiation upon TCDD treatment in mouse C2C12 cells. This TCDD-induced suppression of muscle AChE was proposed to involve an aryl hydrocarbon receptor (AhR)-independent mechanism, but the precise underlying mechanism remains unclear. Considering the widely recognized role of muscular activity in AChE expression and its potential crosstalk with the AhR signaling pathway, we sought to investigate the effect of TCDD on muscle AChE expression in the presence of muscular activity. Therefore, we employed a highly contractile rat primary skeletal muscle culture system in which AChE activity and the expression of genes related to it (AChE T subunit and collagen Q (ColQ)) were increased during the myogenic differentiation process. Although TCDD treatment successfully induced the expression of genes regulated by AhR activation, the treatment exerted no notable effects on myogenic differentiation. Moreover, muscle AChE enzymatic activity and mRNA level remained unchanged following TCDD treatment, and only ColQ mRNA expression was slightly increased after 4-day treatment with TCDD (10(-10) M). The compensatory role of muscle-contraction-related signaling pathways in this newly identified unresponsiveness of muscle AChE to TCDD warrants further investigation. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42530]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Hong Kong Univ Sci & Technol, Ctr Chinese Med, Hong Kong, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 4.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Yali,Xie, Heidi Qunhui,Chen, Yangsheng,et al. Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on acetylcholinesterase during myogenic differentiation of contractile rat primary skeletal muscle cells[J]. CHEMICO-BIOLOGICAL INTERACTIONS,2019,308:164-169. |
| APA | Luo, Yali.,Xie, Heidi Qunhui.,Chen, Yangsheng.,Xia, Yingjie.,Sha, Rui.,...&Zhao, Bin.(2019).Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on acetylcholinesterase during myogenic differentiation of contractile rat primary skeletal muscle cells.CHEMICO-BIOLOGICAL INTERACTIONS,308,164-169. |
| MLA | Luo, Yali,et al."Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on acetylcholinesterase during myogenic differentiation of contractile rat primary skeletal muscle cells".CHEMICO-BIOLOGICAL INTERACTIONS 308(2019):164-169. |
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来源：生态环境研究中心
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