Effects of astrocyte conditioned medium on neuronal AChE expression upon 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure
文献类型：期刊论文
| 作者 | Sha, Rui2; Chen, Yangsheng2; Luo, Yali2; Liu, Yiyun2; Xu, Li2; Xie, Heidi Qunhui2; Zhao, Bin2 |
| 刊名 | CHEMICO-BIOLOGICAL INTERACTIONS
 |
| 出版日期 | 2019-08-25 |
| 卷号 | 309页码:- |
| 关键词 | Dioxin Astrocyte Neuron Astrocyte conditioned medium Acetylcholinesterase |
| ISSN号 | 0009-2797 |
| 英文摘要 | Acetylcholinesterase (EC3.1.1.7; AChE) is a key enzyme in the cholinergic system. Emerging evidence has shown that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a typical persistent organic pollutant, suppressed neuronal AChE activity via dysregulation of different biosynthesis processes in human and rat neuronal cells. In the nervous system, astrocytes protect neurons from environmental pollutants. As a known target cell of TCDD, the astrocyte might be involved in TCDD effects on neuronal AChE. Therefore, in the present study, we found astrocyte-derived conditioned medium (ACM) could induce AChE activity preferentially in mature neurons in the absence of TCDD. The enzymatic activity of AChE was generally decreased in cultured cortical neurons upon direct treatment with TCDD (0.003-0.01 nM). This trend of changes in AChE activity was not significantly altered in immature neurons exposed to ACM produced in the presence of TCDD (TACM group), but reversed in mature neurons. Compared with effects of treatment with ACM plus TCDD (ACMT), a significant differential effect on AChE activity was found in the TACM group in response to TCDD treatment specifically in immature neurons, suggesting the presence of a TCDD-specific active component derived from the astrocyte. Inconsistent alterations in expression and enzymatic activities of the AChE(T) subunit (AChE(T)) and the proline-rich membrane anchor (PRiMA) were found, suggesting that a mechanism of action beyond the transcriptional level might be involved. These data indicate that the astrocyte might play a protective role in TCDD-induced alterations of neuronal AChE in certain stages of differentiation. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42533]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sha, Rui,Chen, Yangsheng,Luo, Yali,et al. Effects of astrocyte conditioned medium on neuronal AChE expression upon 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure[J]. CHEMICO-BIOLOGICAL INTERACTIONS,2019,309:-. |
| APA | Sha, Rui.,Chen, Yangsheng.,Luo, Yali.,Liu, Yiyun.,Xu, Li.,...&Zhao, Bin.(2019).Effects of astrocyte conditioned medium on neuronal AChE expression upon 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure.CHEMICO-BIOLOGICAL INTERACTIONS,309,-. |
| MLA | Sha, Rui,et al."Effects of astrocyte conditioned medium on neuronal AChE expression upon 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure".CHEMICO-BIOLOGICAL INTERACTIONS 309(2019):-. |
入库方式： OAI收割
来源：生态环境研究中心
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